Re: [Histonet] frozen sections

From:Linda M Watson



If they are not embedded in OCT then place them in 10% NBF-fix for 
appropriate amount of time prior to processing.You may have some freezer 
burn around the edges even after fixation but the rest of the tissue 
should be OK. If they are embedded in OCT you can either thaw it out 
remove the tissue as best as you can from the OCT and place the sample 
in 10% NBF(fix and process appropriately). If you want to section the 
frozen first and follow with formalin fixation prior to the IHC that 
sometimes works and gives better morphology than acetone fixation.

Good luck,
Linda

Helen E Johnson wrote:

>I have some frozen, unfixed tissues that I now wish I had fixed so that I
>could section them.  How difficult / possible would it be to cut and fix
>sections from these tissues that would be suitable for immunohistochemistry
>or fluorescence?
>Helen Johnson (hej01@health.state.ny.us)
>
>
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