Way back when I was doing GMA (back in the late 70's and 80's) I placed my section on a DI water bath with NO heat, picked it up with my slide, then placed it on a slide warmer at-least 37 degrees centigrade or higher to adhere the section to the slide.  Make sure you wipe the excess water from the back of the slide, so it doesn't snap crackle pop!

Akemi Allison-Tacha, BS, HT(ASCP)HTL
Client Services Manager
PhenoPath laboratories
551 North 34th Street, Suite 100 
Seattle, WA 98103-8675
Work: (206) 374-9000 ext 1053
E-Mail: akemiat3377@yahoo.com


--- On Wed, 5/28/08, Monfils, Paul  wrote:

> From: Monfils, Paul 
> Subject: [Histonet] flattening glycol methacrylate sections
> To: histonet@lists.utsouthwestern.edu
> Date: Wednesday, May 28, 2008, 10:40 AM
> Do you float your G.M. sections on a water bath?  If so,
> what temperature?  Do you add anything to the water?
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