Fixing the tissue in formalin+alcohol+acetic acid will improve retention 
of HA and other GAGs.

Geoff


Rema wrote:
> -
>
> Hi,
>
> I am trying to stain some rabbit skin to detect Hyaluronic acid but 
> have found that my staining is very weak even though I have left the 
> samples in 1% Alcian blue (pH 2.5) for 30 mins which is what standard 
> procedures from Histo technique books say to do. I am also 
> counterstaining with neutral red. Does anyone have a procedure that 
> knows works or could suggest how I can increase the staining intensity?
>
> Many thanks,
> Rema
>
> ------------------------------------------------------------------------
>
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>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



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