We also use the Richard Allen 7211 but we haven't had any complaints. Maybe it's just a bad batch. Contact the vendor I am sure they will fix it....
Shawn Leslie HT (ASCP)
Scientific Research Manager
University of Florida
School of Veterinary Medicine
352-392-2235 ext 4555
>>> "Angela Bitting" 5/21/2008 3:18 PM >>>
We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
>>> "Gayle Callis" 5/20/2008 4:00 PM >>>
We use xylene to remove coverslips and do this work inside a hood. After
coverslip is off and mounting media is gone with an extra xylene rinse, we
go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try
an minimize exposure to xylene is done. Unfortunately, we found the
substitute just didn't work for this job.
Gayle M. Callis
----- Original Message -----
From: "Patty Dunlop"
Sent: Tuesday, May 20, 2008 1:20 PM
Subject: [Histonet] xylene substitute recoverslipping
> Hello Histonetters,
> I use Clear-Rite 3, a xylene substitute, and am having problems when I
> to recoverslip. Currently, I just put the slide that needs recoverslipped
> into a coplin jar of Clear-rite 3 at room temperature. The coverslip
> doesn't like to come off (takes a week) and it tends to be a sticky, messy
> process. Does anyone have a method for removing coverslips with a xylene
> substitute? Should I use heat?
> Histonet mailing list
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