Yes, I am experiencing the same problem. I haven't changed my protocol, since it's been in place now for the past 6 years. About three weeks ago, my pathologist started complaining that the nuclei is too pale.
I use their 7211 and I asked for a sample of their Hematoxylin 1. I'll see if it produces a darker stain.
---- Angela Bitting wrote:
> We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now.
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
> >>> "Gayle Callis" 5/20/2008 4:00 PM >>>
> We use xylene to remove coverslips and do this work inside a hood. After=20
> coverslip is off and mounting media is gone with an extra xylene rinse, we
> go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try
> an minimize exposure to xylene is done. Unfortunately, we found the
> substitute just didn't work for this job.
> Gayle M. Callis
> Bozeman MT
> ----- Original Message -----
> From: "Patty Dunlop"
> Sent: Tuesday, May 20, 2008 1:20 PM
> Subject: [Histonet] xylene substitute recoverslipping
> > Hello Histonetters,
> > I use Clear-Rite 3, a xylene substitute, and am having problems when I=20
> > need
> > to recoverslip. Currently, I just put the slide that needs recoverslipped
> > into a coplin jar of Clear-rite 3 at room temperature. The coverslip=20
> > really
> > doesn't like to come off (takes a week) and it tends to be a sticky, messy
> > process. Does anyone have a method for removing coverslips with a xylene
> > substitute? Should I use heat?
> > Thanks,
> > Patty
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