Re: [Histonet] Rate of formalin penetration in human brain sections

From:Rene J Buesa

This is one of the less thankful "thank yous" I have read in HistoNet.
  René J. wrote:
  Thank you to all for the information provided. My investigator had already 
located this information and was happy to see that he is on the right 
track with his project. On a side note, I often find this group very 
informative and helpful, but at other times I am baffled by the responses. 
If you believe that a inquiry is due to an individual's laziness, wouldn't 
it be best to either ask for additional information so that you may 
provide "food for thought"; or ignore the question outright?

John Kiernan 
05/28/2008 10:43 PM

Re: [Histonet] Rate of formalin penetration in human brain sections

Dear Karen,

"One of your investigators" needs to visit his library. Perhaps even spend 
$30 of his grant money on on a book or two for the lab. His questions are 
not new. They are addressed well in:

Mann, G. (1902) Physiological Histology. Oxford: Clarendon Press.

Baker, J.R. (1958) Principles of Biological Microtechnique. London: 

Horobin, R.W. (1982) Histochemistry. Stuttgart: Fischer.

These are classics in the field of fixation and staining. 
There are also very recent textbooks in the field. One of them is by me. 

John Kiernan
Anatomy, UWO
London, Canada
= = =
Original Message -----
Date: Tuesday, May 27, 2008 17:18
Subject: [Histonet] Rate of formalin penetration in human brain sections

> Hi--
> One of our investigators is interested in the approximate rate 
> of fixation 
> of human brain tissue, independent of any formaldehyde diffusion 
> effects. 
> In other words, in a very small or very thin piece of human 
> brain tissue, 
> what is the fixation rate? He found published data for rat 
> kidney, but 
> would like to try to at least determine if the fixation rate 
> should be 
> higher or lower in human brain tissue. He is looking for data 
> for room 
> temperature, but any information would be greatly appreciated.
> Thanks!
> Karen M Skish, MS, PA(ASCP)MT
> Pathologists' Assistant & Manager, Neuropathology Lab
> Rush Alzheimer's Disease Center
> Cohn Research Building, Lab 441
> 1735 West Harrison Street
> Chicago IL 60612
> _______________________________________________
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