Dear Karen, "One of your investigators"
n= eeds to visit his library. Perhaps even spend $30 of his grant
money= on on a book or two for the lab. His
questions are= not new. They are addressed well
in: <= BR>Mann, G. (1902) Physiological
Histology. Oxford: Clarendon Press. Baker, J.R. (1958) Principles
of= Biological Microtechnique. London:
Methuen. Horobin, R.W. (1982)
Histochemistry. Stuttgart: Fischer. These are classics in the field of fixation
an= d staining. There are also very recent
textb= ooks in the field. One of them is by me.
&= nbsp;John Kiernan Anatomy,
UWO London, Canada = = =
= ---- Original Message ----- F rom: Karen_Skish@rush.edu Date: Tuesday, May 27,
20= 08 17:18 Subject: [Histonet] Rate of formalin
penetration= in human brain sections To:
histonet@lists.utsouthwestern.= edu
>
Hi-- > One of our investigator= s is interested in the
approximate rate > of fixation <= BR>> of
human brain tissue, independent of any formaldehyde d= iffusion
> effects. > In other words, = in a
very small or very thin piece of human > brain tissu e, > what is the fixation rate? He found published
da= ta for rat > kidney, but > would
like to= try to at least determine if the fixation rate
> should = be > higher or lower in human
brain tissue. He is looki= ng for data > for room
> temperature, bu= t any information would be greatly
appreciated. > Thanks= ! > Karen M
Skish, MS, PA(ASCP)MT > Path= ologists' Assistant
& Manager, Neuropathology Lab &= gt; Rush
Alzheimer's Disease Center > Cohn Research Bu ilding, Lab 441 > 1735 West Harrison
Street >= ; Chicago IL 60612 >
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