Re: [Histonet] Rate of formalin penetration in human brain sections

From:John Kiernan

   Dear Karen,
"One of your investigators" n= eeds to visit his library. Perhaps even spend $30 of his grant money= on on a book or two for the lab.  His questions are= not new.  They are addressed well in:
 <= BR>Mann, G. (1902) Physiological Histology. Oxford: Clarendon Press.
Baker, J.R. (1958) Principles of= Biological Microtechnique. London: Methuen.
 Horobin, R.W. (1982) Histochemistry. Stuttgart: Fischer.
These are classics in the field of fixation an= d staining.
There are also very recent textb= ooks in the field. One of them is by me.
&= nbsp;John Kiernan
Anatomy, UWO
London, Canada
= = = =
Original Message -----
F rom:
Date: Tuesday, May 27, 20= 08 17:18
Subject: [Histonet] Rate of formalin penetration= in human brain sections
To: histonet@lists.utsouthwestern.= edu

> Hi--
> One of our investigator= s is interested in the approximate rate
> of fixation <= BR>> of human brain tissue, independent of any formaldehyde d= iffusion
> effects.
> In other words, = in a very small or very thin piece of human
> brain tissu e,
> what is the fixation rate? He found published da= ta for rat
> kidney, but
> would like to= try to at least determine if the fixation rate
> should = be
> higher or lower in human brain tissue. He is looki= ng for data
> for room
> temperature, bu= t any information would be greatly appreciated.
> Thanks= !
> Karen M Skish, MS, PA(ASCP)MT
> Path= ologists' Assistant & Manager, Neuropathology Lab
&= gt; Rush Alzheimer's Disease Center
> Cohn Research Bu ilding, Lab 441
> 1735 West Harrison Street
>= ; Chicago IL 60612
> ____________ _______________________ 5F= ___________
> Histonet mailing list<= BR>>
> ht tp://
3C= BR>

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