Re: [Histonet] Microwave deparaffinization.

From:"Anne van Binsbergen"



as per the DAKO PT link procedure we have tried dewaxing slides for IHC
using our standard buffers (with tween) and heating them up to the
recommended temps - works like a charm
ER and PR looking good
i could not believe when reading the 'blurb' that the solution used to
'boil' out the wax would not leave a greasy residue on the slides as they
are removed and cooler air congeals the waxy/buffer on the slides
but i was wrong - try it out
Annie

2008/5/21 Tony Henwood :

> Sebastian,
> The following article uses a heated detergent solution to dewax and
> rehydrate sections. I have not as yet used it myself but I plan to try it at
> some time.
>
> Lars Falkeholm, Crawford A. Grant, Anders Magnusson, and Eva Möller (2001)
> "Xylene-Free Method for Histological Preparation: A Multicentre Evaluation"
> LABORATORY INVESTIGATION Vol. 81, No. 9, p. 1213-1221.
>
>
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: 612 9845 3306
> Fax: 612 9845 3318
>
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebastián Vecchio
> Sent: Tuesday, 20 May 2008 5:36 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Microwave deparaffinization.
>
>
> I have been trying to deparafinize section in the lab, but I cannot  get
> the right time and  power for the MW oven, does any one has a protocol for
> this . This method eliminates the uses  of xylol in the processing of
> staining sections. Thanks. Sebastian, Student of Histotechnology, EUTM,
> FMED, UDELAR. _______________________________________________
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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