Re: [Histonet] MMA de-plasticizing

From:mwstarbu@mdanderson.org



Hi Michele,

We use 2-methoxyethyl acetate  a.k.a. 1-acetoxy-2-methoxyethane (AME).  We 
do 2 changes 20 minutes each at room temperature under the hood.  This 
works very well for us.

Good luck,

Mike

 





Michele Wich 
Sent by: 
05/30/2008 10:39 AM



 

To:

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Subject:
[Histonet] MMA de-plasticizing



Is there anyone out there doing MMA on un-decalcified bone? I'm wondering 
if there is any way to de-plasticize without putting the slides in heated 
xylene--a potentially explosive situation which technically would require 
explosion proof oven, hood, clothing, etc. I know that the flash point of 
xylene is quite low (26.1C, I think, which is barely above room 
temperature). Is there a way around this safety issue?


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