Re: [Histonet] HIER to repair dried specimens?

From:"Bryan Hewlett"

It's called re-hydration!
Similar techniques are used for partial restoration of mummified tissue for 
histological examination.


----- Original Message ----- 
Sent: Thursday, May 22, 2008 10:49 AM
Subject: [Histonet] HIER to repair dried specimens?

>I have accidentally stumbled on an unexplainable "by product" of HIER in 
>citrate buffer, pH6.0.
> We had some specimens that had unfortunately air dried prior to fixation 
> and processing and therefore looked terrible. The H&E was practically 
> unreadable due to drying artifact. In an effort to see something 
> worthwhile, IHC stains were ordered. The hematoxylin counterstain, 
> following retrieval showed much better nuclear detail than the original 
> H&E. Several additional slides were cut and stained with H&E following 
> HIER. They were much better. This has been repeated on a few other 
> specimens with similar resu
> lts. Any ideas as to how or why this is working?
> thanks
> sc
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