It's called re-hydration!
Similar techniques are used for partial restoration of mummified tissue for
----- Original Message -----
Sent: Thursday, May 22, 2008 10:49 AM
Subject: [Histonet] HIER to repair dried specimens?
>I have accidentally stumbled on an unexplainable "by product" of HIER in
>citrate buffer, pH6.0.
> We had some specimens that had unfortunately air dried prior to fixation
> and processing and therefore looked terrible. The H&E was practically
> unreadable due to drying artifact. In an effort to see something
> worthwhile, IHC stains were ordered. The hematoxylin counterstain,
> following retrieval showed much better nuclear detail than the original
> H&E. Several additional slides were cut and stained with H&E following
> HIER. They were much better. This has been repeated on a few other
> specimens with similar resu
> lts. Any ideas as to how or why this is working?
> Histonet mailing list
Histonet mailing list