Yes sorry my message was assuming your primaries were from different
species, otherwise indeed you need to use specialized blocking
methods ... I think I am too tired to be emailing clearly!!!
> you need to block the 1st primary antibody with IgG, and then use
>appropriate serum, before using another primary from the same species.
>I would use a different primary AB from a different species, i.e. if u used
>goat anti.. The 1st time (when mistake was made) now use rabbit anti..
>If you have to use another primary from the same species - I suggest review
>excellent protocols on jacksonimmuno.com (labeling with 2 primaries from the
>same species). This works, although may give you some background if blocking
>is not done properly.
>Dr. Igor O. Nasonkin
>National Institutes of Health/NEI
>9000 Rockville Pike, MSC 1864
>Bldg 10, Room 10B11
>Bethesda, MD 20892
>Tel: 301-443-7398 -work
> 617-388-4104 -cell
>------ Forwarded Message
>> Date: Tue, 20 May 2008 03:16:16 +0000
>> To: Linke_Noelle ,
>> , "Mejia, Maria"
>> Subject: Re: [Histonet] one heck of a IHC question regarding
>> fixedfree-floating brain sections
>------ End of Forwarded Message
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