I have worked with animal tissues for well over 18 years and I agree
with other previous posters here, that your problem is most likely the
fixation at 4oC.
Dusko Trajkovic


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaupp,
Dina D 
Sent: Wednesday, June 04, 2008 4:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] mouse heart chatter upon sectioning...

Whenever I process (whole)mouse hearts, the tissue chatters upon
sectioning.  The tissue is usually fixed overnight in 10%nbf at 4C
before processed.  I tried different processing times.  The last time I
processed hearts, I processed them for 5 hours total no vaccum.  Should
I decrease the processing time even lower than that?  I am afraid of
underprocessing.  I have to section the entire heart from top to bottom,
so I really need some advice.
 
(open system) Processor Set Up:
1 - fix (if tissue fixed overnight, skip #1 & #2 stations)
2 - fix
3 - 70%
4 - 80%
5 & 6 - 95%
7 & 8 - 100%
9 & 10- Xylene Sub. - Clear-rite
11 & 12-Paraffin stations
 
Thanks,
Dina
dgaupp@tulane.edu  
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