Several queries,
Why fix in the fridge? Do you want to slow down the fixation or
introduce ice crystal artifact?
If the tissue is shattering, one of the problems might be the fact that
the tissue is fixed in ethanol reather than formalin.
What are the processing times & temperatures.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaupp,
Dina D
Sent: Thursday, 5 June 2008 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] mouse heart chatter upon sectioning...
Whenever I process (whole)mouse hearts, the tissue chatters upon
sectioning. The tissue is usually fixed overnight in 10%nbf at 4C
before processed. I tried different processing times. The last time I
processed hearts, I processed them for 5 hours total no vaccum. Should
I decrease the processing time even lower than that? I am afraid of
underprocessing. I have to section the entire heart from top to bottom,
so I really need some advice.
(open system) Processor Set Up:
1 - fix (if tissue fixed overnight, skip #1 & #2 stations)
2 - fix
3 - 70%
4 - 80%
5 & 6 - 95%
7 & 8 - 100%
9 & 10- Xylene Sub. - Clear-rite
11 & 12-Paraffin stations
Thanks,
Dina
dgaupp@tulane.edu
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