RE: [Histonet] heat + formalin

From:"Juan Gutierrez"

Hi Nancy, if you are doing breast prognostic markers on your cases, you
might want to let it fix overnight before processing.  The FDA requires it
for Her-2 testing.  No we do not use heat on our formalin steps, but we
require our docs to cut tissue at 3mm or less thickness. So far this has
been working fine with fixing and infiltration.

Good luck,

Juan C. Gutierrez, HT(ASCP)
Precision Pathology Services

-----Original Message-----
[] On Behalf Of Nancy
Sent: Wednesday, May 28, 2008 9:09 AM
To: ''
Subject: [Histonet] heat + formalin

Good Morning

Need some input, please.  Does anyone combine heat during the formalin part
of processing?  We are having some issues with breast cases that need to be
read out the next am - we are running them on a shorter processing schedule
(done at 4 am) - and not having the best results.We use a closed system
Tissue-Tek VIP 3000.  Is anybody using microwave fixation?
 I think we will just put them on the longer processor (done at 0630) and be
done - but wanted to check for other options. 

Thanks in advance
Nancy Schmitt
Histology Coordinator
Dubuque, IA

NOTICE: This email may contain legally privileged information. The
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the sender
that you have received it in error and then delete it along with any
attachments. Thank you.

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>