RE: [Histonet] Richard Allan Hematoxylin problems

From:"Mary Abosso"

I was the QA Histotechnologist at Richard-Allan and the best way to handle any product issues is to call in to Tech-Rite technical support who can help figure out what the issue is.  Additonally, they will file a complaint with the QA department who will thouroughly investigate the retain sample from that lot, all of the chemicals used in the batch.  Richard-Allan (Thermo-Fisher Scientific) is committed to the highest standard of quality and needs the customer feed back to continuously improve.
Mary Abosso
Unipath LLC.
Denver, CO


From: on behalf of Shawn Leslie
Sent: Wed 5/21/2008 2:30 PM
To: Angela Bitting
Cc: HistoNet
Subject: Re: [Histonet] Richard Allan Hematoxylin problems

We also use the Richard Allen 7211 but we haven't had any complaints. Maybe it's just a bad batch. Contact the vendor I am sure they will fix it....

Shawn Leslie  HT (ASCP)
Scientific Research Manager
Anatomic Pathology
University of Florida
School of Veterinary Medicine
352-392-2235 ext 4555

>>> "Angela Bitting"  5/21/2008 3:18 PM >>>
We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now.

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

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However many electrons were severly inconvienienced!

>>> "Gayle Callis"  5/20/2008 4:00 PM >>>
We use xylene to remove coverslips and do this work inside a hood.  After
coverslip is off and mounting media is gone with an extra xylene rinse, we
go back to Clearite 3 to rinse off xylene and recoverslip.  Anything to try
an minimize exposure to xylene is done.  Unfortunately, we found the
substitute just didn't work for this job.

Gayle M. Callis
Bozeman MT

----- Original Message -----
From: "Patty Dunlop" 
Sent: Tuesday, May 20, 2008 1:20 PM
Subject: [Histonet] xylene substitute recoverslipping

> Hello Histonetters,
> I use Clear-Rite 3, a xylene substitute, and am having problems when I
> need
> to recoverslip.  Currently, I just put the slide that needs recoverslipped
> into a coplin jar of Clear-rite 3 at room temperature.  The coverslip
> really
> doesn't like to come off (takes a week) and it tends to be a sticky, messy
> process.  Does anyone have a method for removing coverslips with a xylene
> substitute?  Should I use heat?
> Thanks,
> Patty
> _______________________________________________
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