SUMMARY
It is shown by sedimentation velocity measurements that the
presence of undissociated acetic acid favors the dissociation of
hemoglobin in a pH region in which hydrogen ion had previously
been thought to have the dominant effect.
Acknowledgments-We wish to acknowledge continuous discussion
and collaboration with Professors J. Wyman and E. Antonini
during the course of this work.
REFEREYCES
1. REITHEL, F. J., Advances in Protein Chem., 18, 123 (1963).
2. PEDERSEN, K. O., AND ANDERSSON, K. J. I., in T. SVEDBERG
AND K. 0. PEDERSEN (Editors), The ultracentrifuge, Oxford
University Press, London, 1940, p. 407.
3. ROSSI-FANELLI, A., ANTONINI, E., AND CAPUTO, A., Advances
in Protein Chem., 19, 73 (1964).
4. PERUTZ, M. F., Biochem. J., 94, 21P (1965).
5. CULLIS, A. F., MUIRHEAD, H., PERUTZ, M. F., ROSSMANN,
M. G., AND NORTH, A. C. T., Proc. Roy. Sot. London, Ser. A,
265, 161 (1962).
6. PHELPS, R. A., AND CANN, J. R., J. Am. Chem. Sot., 78, 3539
(1956).
7. CANN, J. R., J. Biol. Chem., 235, 2810 (1960).
8. CANN, J. R., AND GOAD, W. B., J. Biol. Chem., 240, 148 (1965).
9. FIELD, E. O., AND O'BRIEN, J. R., Bioehem. J., 60, 656 (1955).
10. ROSSI-FANELLI, A., ANTONINI, E., AND CAPUTO, A., J. Biol.
Chem., 236, 391 (1961).
11. LONG, C. (Editor), Biochemists' handbook, Spon Ltd., London,
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12. PEDERSEN, K. O., J. Phys. Chem., 62, 1282 (1958).
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: 28 May 2008 05:10
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reaction between Haemoglobin and acetic acid
Hi all,
I have been asked what is the altered haemoglobin called that results
from the treatment of tissues with acetic acid.
All I could think of was that carbon monoxide reacts with haemoglobin to
form carboxyhemoglobin.
Any ideas?
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
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