RE: [Histonet] Re: Disposal of Tissue blocks

From:"Mickie Johnson"



We sent them to an incinerator through bio-hazard trash pick-up when I was
supervising our hospital lab.

Best Regards,
 
Mickie
 
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX   509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com 
Email: mickie25@netzero.net
 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris
Evanish
Sent: Wednesday, June 04, 2008 9:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Disposal of Tissue blocks

Can I have your inputs on the method you use to dispose of old tissue
paraffin blocks


>>>  6/4/2008 9:05 am >>>
Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
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Today's Topics:

   1. RE: Histonet Digest, Vol 55, Issue 3 (Jerry Helisek)
   2. Re: Toluidine Blue and H&E (Robert Richmond)
   3. Plastic coplin Jar with Domed Lid (Ellenburg, Deborah)
   4. Alternative to Histobath (Melissa Gonzalez)
   5. Correlation studies (Clark Stephen - Myrtle Beach)
   6. traveling (Karla Arrington)
   7. Employment Opportunities (Tawanda Johnson)
   8. Re: Correlation studies (Rene J Buesa)
   9. xylene grades (Merced Leiker)
  10. Re: Correlation studies (Joe Nocito)
  11. RE: MMA de-plasticizing (Michelle McDonald)
  12. Re: dome lid coplin jars (Ale? Kladnik)
  13. Re: Toluidine Blue and H&E (Jim Reilly)
  14. RE: Alternative to Histobath (Alan Bright)
  15. GMA immunos (Edwards, R.E.)
  16. RE: Creative Waste Solutions (anitathorn@comcast.net)
  17. RE: MLT working histology? (Tom McNemar)
  18. Re: xylene grades (Rene J Buesa)
  19. RE: MLT working histology? (Houston, Ronald)
  20. RELIA Special JOB Alert (Pam Barker)
  21. ATPase. (Ian Montgomery)
  22. RE: MLT working histology? (Whitaker, Bonnie)


----------------------------------------------------------------------

Message: 1
Date: Tue, 3 Jun 2008 13:11:46 -0400
From: "Jerry Helisek" 
Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 3
To: 
Message-ID:
	<3855F92002259948A66A8CA2D16E3A4F0B544E@server.ralambusa.com>
Content-Type: text/plain;	charset="us-ascii"

Sandy:

This may work for you... See:
http://www.ralamb.net/product_info.php?products_id=347 

Thanks

------------------------------------

Raymond A. Lamb, Inc

Jerry Helisek

VP North America

jerry@ralambusa.com 

5409 Lumley Road, Unit 102

Durham, North Carolina 27703

tel: 919.957.1964

fax: 919.957.1972

mobile: 919.264.7964

Skype ID:jerryhelisek

------------------------------------



Message: 1
Date: Mon, 2 Jun 2008 12:19:57 -0500
From: "Harrison, Sandra C." 
Subject: [Histonet] dome lid coplin jars
To: 
Message-ID:
	

Content-Type: text/plain;	charset="us-ascii"

This is a small thing, but it would make my Pathology People so happy
(and me too!!) if I could order some plastic coplin jars with the
domed
lid.  Has anyone received any recently and if so, which supplier did
you
use and catalog number?

I've tried Cardinal and Fisher with no luck.  Also Electron Microscopy
Sciences.  They send the flat lidded, plastic coplin jar that is too
deep.

The problem with the flat lidded, plastic coplin jars we have received
is that we can't pull slides out with just our gloved fingers, but
must
instead use forceps, because they are so deep.  The catalogs show a
picture of a domed lid, but they send you the flat lidded coplin jar. 


Any suggestions?

Thanks,
Sandy



------------------------------

Message: 2
Date: Tue, 3 Jun 2008 13:20:50 -0400
From: "Robert Richmond" 
Subject: [Histonet] Re: Toluidine Blue and H&E
To: histonet@lists.utsouthwestern.edu 
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Erin Martin asks: >>My pathologist would like to do
 a combined
toluidine blue and H&E. Has anyone ever done this? If so, would you
mind sharing your procedure? I am guessing that I would have to use
the toluidine blue after the eosin then air dry the slides since
alcohol would take the blue out, but I am not sure if it would react
with either the hematoxylin or the eosin.<<

What an interesting idea! I suppose we're talking about staining
Helicobacter in gastric biopsy specimens. I was one of the early users
of toluidine blue for Helicobacter, and I've more or less followed the
literature but have never found such a technique. As others have
suggested, I suppose the toluidine blue would come after the H & E.
Moving the slides rapidly through alcohols would probably keep the dye
from washing out. Most people would probably use Diff-Quik II (or a
generic equivalent) rather than preparing toluidine blue.

The only way to find out is to try it! I'd want you to have good
control sections to practice with, and I'd expect to work closely with
the histotechnologist to get the desired result. I hope somebody does
try this and report back to us all.

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

Message: 3
Date: Tue, 3 Jun 2008 13:44:47 -0400
From: "Ellenburg, Deborah" 
Subject: [Histonet] Plastic coplin Jar with Domed Lid
To: 
Message-ID:
	
Content-Type: text/plain;	charset=iso-8859-1




Sandy,

I went online and searched for domed lid coplin jars and came up with
the following.

Thermo Scientific - Shandon Plastic Coplin Staining Jar - SKU:  194
Hope this helps.

Debbie E.



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu 
Sent: Tuesday, June 03, 2008 1:07 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 55, Issue 3

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu 

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
	histonet-owner@lists.utsouthwestern.edu 

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. dome lid coplin jars (Harrison, Sandra C.)
   2. RE: dome lid coplin jars (Pat.Bell@UCHSC.edu)
   3. Re: dome lid coplin jars (Kathleen Roberts)
   4. bausch surgical (Emily Sours)
   5. enzyme histochemistry- automation (Joanne Mauger)
   6. Toluidine Blue and H&E (Martin, Erin)
   7. Re: Toluidine Blue and H&E (Rene J Buesa)
   8. Trying to reach Tracey Lenek (Pam Barker)
   9. Histological products (Myl?ne de Champlain)
  10. Re: Histological products (Rene J Buesa)


----------------------------------------------------------------------

Message: 1
Date: Mon, 2 Jun 2008 12:19:57 -0500
From: "Harrison, Sandra C." 
Subject: [Histonet] dome lid coplin jars
To: 
Message-ID:
	

Content-Type: text/plain;	charset="us-ascii"

This is a small thing, but it would make my Pathology People so happy
(and me too!!) if I could order some plastic coplin jars with the
domed
lid.  Has anyone received any recently and if so, which supplier did
you
use and catalog number?

I've tried Cardinal and Fisher with no luck.  Also Electron Microscopy
Sciences.  They send the flat lidded, plastic coplin jar that is too
deep.

The problem with the flat lidded, plastic coplin jars we have received
is that we can't pull slides out with just our gloved fingers, but
must
instead use forceps, because they are so deep. 
 The catalogs show a
picture of a domed lid, but they send you the flat lidded coplin jar. 


Any suggestions?

Thanks,
Sandy


------------------------------

Message: 2
Date: Mon, 2 Jun 2008 11:23:01 -0600
From: 
Subject: RE: [Histonet] dome lid coplin jars
To: ,	
Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3C023@java.uchsc.edu>
Content-Type: text/plain;	charset="US-ASCII"

Try Market Lab and VWR.

Pat Bell HT(ASCP)
Sr. PRA
Division of Medical Oncology
UCHSC, Aurora, CO
303-724-3845 (lab)
303-724-3889 (fax)
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Harrison, Sandra C.
Sent: Monday, June 02, 2008 11:20 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] dome lid coplin jars

This is a small thing, but it would make my Pathology People so happy
(and me too!!) if I could order some plastic coplin jars with the
domed
lid.  Has anyone received any recently and if so, which supplier did
you
use and catalog number?

I've tried Cardinal and Fisher with no luck.  Also Electron Microscopy
Sciences.  They send the flat lidded, plastic coplin jar that is too
deep.

The problem with the flat lidded, plastic coplin jars we have received
is that we can't pull slides out with just our gloved fingers, but
must
instead use forceps, because they are so deep.  The catalogs show a
picture of a domed lid, but they send you the flat lidded coplin jar. 


Any suggestions?

Thanks,
Sandy
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



------------------------------

Message: 3
Date: Mon, 02 Jun 2008 13:41:13 -0400
From: Kathleen Roberts 
Subject: Re: [Histonet] dome lid coplin jars
To: "Harrison, Sandra C." 
Cc: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: <484430B9.9000302@rci.rutgers.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

How about MarketLab?  www.marketlabinc.com   Cat# ML9801 

I'm about to order some myself, but before I do so, are these safe to 
use in the microwave?  I think so, but I want to ask and be sure.

-Kathy Roberts
Principal Lab Technician
Neurotoxicology Laboratories
Dept of Pharmacology and Toxicology
Ernest Mario School of Pharmacy
Rutgers, the State University of NJ
41 B Gordon Rd
Piscataway, NJ 08854





Harrison, Sandra C. wrote:

>This is a small thing, but it would make my Pathology People so happy
>(and me too!!) if I could order some plastic coplin jars with the
domed
>lid.  Has anyone received any recently and if so, which supplier did
you
>use and catalog number?
>
>I've tried Cardinal and Fisher with no luck.  Also Electron
Microscopy
>Sciences.  They send the flat lidded, plastic coplin jar that is too
>deep.
>
>The problem with the flat lidded, plastic coplin jars we have
received
>is that we can't pull slides out with just our gloved fingers, but
must
>instead use forceps, because they are so deep.  The catalogs show a
>picture of a domed lid, but they send you the flat lidded coplin jar. 

>
>Any suggestions?
>
>Thanks,
>Sandy
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu 
>http://lists.utsouthwestern.edu/mailman/listinfo/histon 
>  
>



------------------------------

Message: 4
Date: Mon, 2 Jun 2008 13:36:54 -0400
From: "Emily Sours" 
Subject: [Histonet] bausch surgical
To: histonet@lists.utsouthwestern.edu 
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

Hello

We need to sharpen our Vannas scissors, which we usually do through
Storz.
Unfortunately, we haven't done this for about four
 years, and Storz is
now
part of Bausch Surgical.  Their website doesn't have any information
on
where to send them and I've been on hold for ten minutes twice without
reaching anyone.
Does anyone here have their recent address or a non-toll free number
for
them?

Thanks,
Emily
-- 
When you're riding in a time machine way far into the future, don't
stick
your elbow out the window, or it'll turn into a fossil.


------------------------------

Message: 5
Date: Mon, 02 Jun 2008 15:41:59 -0400
From: "Joanne Mauger" 
Subject: [Histonet] enzyme histochemistry- automation
To: ,
	
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Hi Everyone,

Does anyone know of an automated platform for staining fresh(frozen)
muscle slides for ATP, NADH, COX, etc.? I know it's a long shot, but I
had to ask.

Thanks,

Jo



------------------------------

Message: 6
Date: Mon, 2 Jun 2008 13:28:15 -0700
From: "Martin, Erin" 
Subject: [Histonet] Toluidine Blue and H&E
To: "histonet" 
Message-ID:
	
Content-Type: text/plain; charset=iso-8859-1

Hi all,
 
My pathologist would like to do a combined toluidine blue and H&E.  Has
anyone ever done this?  If so, would you mind sharing your procedure?  I
am guessing that I would have to use the toluidine blue after the eosin
then air dry the slides since alcohol would take the blue out, but I am
not sure if it would react with either the hematoxylin or the eosin.  
 
Thank you for your help!
 
Erin Martin




------------------------------

Message: 7
Date: Mon, 2 Jun 2008 13:43:45 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Toluidine Blue and H&E
To: "Martin, Erin" ,	histonet
	
Message-ID: <372902.76788.qm@web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I would try it first without the hematoxylin and before the eosin.
  You don't have to air dry the sections, just blot them and place them
in isopropanol before going to xylene.
  Toluidin blue works better at pH 11 that you can reach with borax
before dissolving the dye in it. It works also better at 50-60ºC.
  Hope this will help you.
  René J.

"Martin, Erin"  wrote:
  Hi all,

My pathologist would like to do a combined toluidine blue and H&E. Has
anyone ever done this? If so, would you mind sharing your procedure? I
am guessing that I would have to use the toluidine blue after the eosin
then air dry the slides since alcohol would take the blue out, but I am
not sure if it would react with either the hematoxylin or the eosin. 

Thank you for your help!

Erin Martin


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


       

------------------------------

Message: 8
Date: Mon, 2 Jun 2008 16:46:52 -0400
From: "Pam Barker" 
Subject: [Histonet] Trying to reach Tracey Lenek
To: 
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

Hi Tracey,

 I tried to respond to your e-mail but the address that I am replying
to is
bouncing back as undeliverable.  The answer to your question is yes and
when
you get a chance please send me a new e-mail address or phone number
to
reach you.  Thanks-Pam

 

Thank You!

 

Pam M. Barker

President

Relia

5703 Red Bug Lake Road #330

Winter Springs, FL 32708-4969

Phone: (407)657-2027

Cell:    (407)353-5070

FAX:    (407)678-2788

Toll Free: (866)607-3542

e-mail: relia1@earthlink.net 

http://home.earthlink.net/~relia1 

www.myspace.com/pamatrelia 

 




------------------------------

Message: 9
Date: Tue, 3 Jun 2008 08:56:20 -0400
From: Myl?ne de Champlain 
Subject: [Histonet] Histological products
To: 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"


Hi,
 
I would like to know if those histological products still good even if
they are expired. They still sealed in the original package. 
 
Products                                          Expiration
Consul-mount histology formulation    09-2007
Shandon xylene-substitute mountant   01-2007
Instant hematoxylin (powder)             09-2003
 
Thank you for your answers.
 
Mylène 
Université du Québec à Rimouski
_________________________________________________________________



------------------------------

Message: 10
Date: Tue, 3 Jun 2008 06:14:56 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Histological products
To: "Mylène" de Champlain ,
	histonet@lists.utsouthwestern.edu 
Message-ID: <36234.55354.qm@web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

If sealed in their original package (not exposed to air), they probably
are.
  On the other hand, it will be always cheaper to prepare a hematoxylin
solution to determine how it works, than to buy a new powder bottle.
  René J.

Mylène de Champlain  wrote:
  
Hi,

I would like to know if those histological products still good even if
they are expired. They still sealed in the original package. 

Products Expiration
Consul-mount histology formulation 09-2007
Shandon xylene-substitute mountant 01-2007
Instant hematoxylin (powder) 09-2003

Thank you for your answers.

Mylène 
Université du Québec à Rimouski
_________________________________________________________________

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


       

------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

End of Histonet Digest, Vol 55, Issue 3
***************************************

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------------------------------

Message: 4
Date: Tue, 3 Jun 2008 11:31:25 -0700
From: "Melissa Gonzalez" 
Subject: [Histonet] Alternative to Histobath
To: 
Message-ID:
	<2884B897182A1D438C7BA24B9A8F94A2348005@hqsvr01mail.cgi.com>
Content-Type: text/plain;	charset="iso-8859-1"

Anyone have any recommendations on tissue freezing units? 

Thanks!

Melissa

 

Melissa A. González Edick

R&D, Cell Genesys, Inc

500 Forbes Blvd

South San Francisco, CA 94080

ph (650) 266-3168

fx  (650) 266-3080

www.cellgenesys.com 

 

 



------------------------------

Message: 5
Date: Tue, 3 Jun 2
008 13:31:39 -0500
From: "Clark Stephen - Myrtle Beach"
	
Subject: [Histonet] Correlation studies
To: 
Message-ID:
	<28E40C736ED32341BBB2B094EFD23FC8020F070B@NASEV05.hca.corpad.net>
Content-Type: text/plain;	charset="us-ascii"

I need to perform correlation studies for our new Immunohistochemistry
machine.  The question I have is what format do I use?  How do I do
it?
What should the test look like?  Etc.  Any advice would be
appreciated.

Thanks,

 

Steve Clark



------------------------------

Message: 6
Date: Tue, 3 Jun 2008 12:22:36 -0700 (PDT)
From: Karla Arrington 
Subject: [Histonet] traveling
To: histonet@lists.utsouthwestern.edu 
Message-ID: <951179.57551.qm@web32501.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Fellow Histo's:

I was wondering if anyone has done the "traveling Histotech" thing?  Is
it worth it? 
Need a change of pace...  Very interested!

Thanks!
Karla Arrington, HT(ASCP)
freckles9660@yahoo.com 



      

------------------------------

Message: 7
Date: Tue, 3 Jun 2008 15:23:53 -0400
From: "Tawanda Johnson" 
Subject: [Histonet] Employment Opportunities
To: 
Message-ID:
	<24D22DE9E488AA43BF92A4389F2DDB1F02BB2C07@mail1.BOSTWICK.COM>
Content-Type: text/plain;	charset="us-ascii"

Bostwick Laboratories are currently seeking experienced Histotech's
for
our Ohio and Connecticut locations.  If you have at least 2years
laboratory experience and a Bachelor in Science Degree please email me
your Resume.  ASCP certification preferred.  

 

 

 



------------------------------

Message: 8
Date: Tue, 3 Jun 2008 12:59:37 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Correlation studies
To: Clark Stephen - Myrtle Beach ,
	histonet@lists.utsouthwestern.edu 
Message-ID: <408291.80171.qm@web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Correlations are always between two sets of variables (unless they
refer to multiple correlations where those sets contain more than two).
  I have never heard of correlating an IHC machine. What are you going
to correlate it with?
  I do not imagine how you are going to present the problem. Are you
trying to correlate your results with your IHC machine with the results
in another or with some other results?
  If that is the case you will have to run both sets simultaneously.
  On the other hand those correlation tests (the strongest) have to
have a normal distribution of the data, or you will have to use some
type of transformation.
  Perhaps you could find more information in
  http://www.vassar.edu/lowry which is a fantastic web site to perform
statistical tests.
  René J.

Clark Stephen - Myrtle Beach  wrote:
  I need to perform correlation studies for our new
Immunohistochemistry
machine. The question I have is what format do I use? How do I do it?
What should the test look like? Etc. Any advice would be appreciated.

Thanks,



Steve Clark

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


       

------------------------------

Message: 9
Date: Tue, 03 Jun 2008 16:59:52 -0400
From: Merced Leiker 
Subject: [Histonet] xylene grades
To: histonet@lists.utsouthwestern.edu 
Message-ID: <8E75CCAA47872DF1593BF16B@bchwxp2702.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed

Would the grade of xylenes used for deparaffinizing make a difference
for 
immunostaining of certain (picky) epitopes?  We are using Certified
A.C.S. 
xylenes.

What about rinsing in tap water vs. distilled water during
rehydration?

The reason I'm asking is that we are having issues staining for c-kit
(KIT, 

CD117) on FFPE tissue.   We've tried all different antigen retrievals,

including no retrieval, and still aren't seeing any stain.

Any help would be greatly appreciated.  Thanks!


Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725




------------------------------

Message: 10
Date: Tue, 3 Jun 2008 18:55:08 -0500
From: "Joe Nocito" 
Subject: Re: [Histonet] Correlation studies
To: "Clark Stephen - Myrtle Beach" ,
	
Message-ID: <003e01c8c5d5$4167d990$0302a8c0@yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Steve,
if you have been performing immunos manually, you can test the machine

results against the manual ones. Run slides manually and on the
machine. 
Reagents should be the same lot number. When I switched to an automated

machine, I had to test each antibody and each HIER method. I kept the 
records and slides for 2 CAP cycles, a bit anal, but I wanted to be
sure 
that CAP understood that I did have a validation system. Good luck

Joe
----- Original Message ----- 
From: "Clark Stephen - Myrtle Beach"

To: 
Sent: Tuesday, June 03, 2008 1:31 PM
Subject: [Histonet] Correlation studies


I need to perform correlation studies for our new Immunohistochemistry
machine.  The question I have is what format do I use?  How do I do
it?
What should the test look like?  Etc.  Any advice would be
appreciated.

Thanks,



Steve Clark

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 11
Date: Wed, 4 Jun 2008 14:20:26 +1000
From: "Michelle McDonald" 
Subject: RE: [Histonet] MMA de-plasticizing
To: "Michele Wich" ,
	
Message-ID: <47BD6E7614A693499453835D47E36F70045F0AD8@hedwig.nch.kids>
Content-Type: text/plain; charset="iso-8859-1"

Dear Michelle,

We de-plasticise MMP bone sections using two changes of 100% acetone
for 10 monis each.

Thanks

Michelle

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Michele Wich
Sent: Saturday, 31 May 2008 1:40 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] MMA de-plasticizing


Is there anyone out there doing MMA on un-decalcified bone? I'm
wondering if there is any way to de-plasticize without putting the
slides in heated xylene--a potentially explosive situation which
technically would require explosion proof oven, hood, clothing, etc. I
know that the flash point of xylene is quite low (26.1°C, I think, which
is barely above room temperature). Is there a way around this safety
issue?


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in error should notify the sender immediately and delete it from his or
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otify the sender.

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------------------------------

Message: 12
Date: Wed, 04 Jun 2008 09:52:54 +0200
From: Ale? Kladnik 
Subject: [Histonet] Re: dome lid coplin jars
To: histonet@lists.utsouthwestern.edu 
Message-ID: <484649D6.6080807@bf.uni-lj.si>
Content-Type: text/plain; charset=UTF-8; format=flowed

Try Bel-Art, they used to sell dome shaped coplin jars, now they claim

they have an improved design with large opening - they even show a 
gloved hand taking a slide in/out of the jar.

http://www.belart.com/cat/lifescience/en/442081000.asp 


> This is a small thing, but it would make my Pathology People so
happy
> (and me too!!) if I could order some plastic coplin jars with the
domed
> lid.  Has anyone received any recently and if so, which supplier did
you
> use and catalog number?
> 
> I've tried Cardinal and Fisher with no luck.  Also Electron
Microscopy
> Sciences.  They send the flat lidded, plastic coplin jar that is too
> deep.
> 
> The problem with the flat lidded, plastic coplin jars we have
received
> is that we can't pull slides out with just our gloved fingers, but
must
> instead use forceps, because they are so deep.  The catalogs show a
> picture of a domed lid, but they send you the flat lidded coplin jar.
 
> 
> Any suggestions?
> 
> Thanks,
> Sandy


-- 
Aleš Kladnik
Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo
VeÄ na pot 111, SI-1000 Ljubljana, Slovenija
tel: +386 1 4233388, fax: +386 1 2573390
url: http://botanika.biologija.org/kdosmo/aleskl.php 
url: http://web.bf.uni-lj.si/bi/mikroskopija/ 



------------------------------

Message: 13
Date: Wed, 4 Jun 2008 09:46:12 +0100
From: "Jim Reilly" 
Subject: [Histonet] Re: Toluidine Blue and H&E
To: 
Message-ID:
	
<82B54C1E04D94D489448735E6CBDE4606DC2B5@exchange-be5.centre.ad.gla.ac.uk>
	
Content-Type: text/plain;	charset="US-ASCII"

Hello 
 
I Tried Toluidine Blue / Eosin on decalcified mouse footpads : 2
minutes
Tol Blue 1 minute Eosin rapid dehydration in 95% alcohol x1 100% x2
(10
dips each) clear in xylene and mount in DPX.   This seemed to work OK.
 
Jim Reilly
 
Glasgow Biomedical Research Centre


------------------------------

Message: 14
Date: Wed, 4 Jun 2008 11:39:23 +0100
From: "Alan Bright" 
Subject: RE: [Histonet] Alternative to Histobath
To: "Melissa Gonzalez" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Dear Melissa,

Yes, we manufacture the Clini-RF Rapid Freezer which goes down to -80
degs. (See our Web site) C. We also fit as an option a Quick Freezer
into our OTF5000 Cryostat.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com 
Web Site: www.brightinstruments.com 
Skype: dazzle0


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Melissa Gonzalez
Sent: 03 June 2008 19:31
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Alternative to Histobath

Anyone have any recommendations on tissue freezing units? 

Thanks!

Melissa

 

Melissa A. González Edick

R&D, Cel
l Genesys, Inc

500 Forbes Blvd

South San Francisco, CA 94080

ph (650) 266-3168

fx  (650) 266-3080

www.cellgenesys.com 

 

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 15
Date: Wed, 4 Jun 2008 12:33:23 +0100
From: "Edwards, R.E." 
Subject: [Histonet] GMA immunos
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<7722595275A4DD4FA225B92CDBF174A172A577C87E@EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"



After a lifetime of immunostaining paraffin wax sections, in my new job
I am having to  deal  with  GMA sections. Two questions, firstly, does
one  have to float out the sections for a minimum of 90 seconds on 0.5%
ammonium hydroxide before mounting on a slide;(  with the  ammonaical
water apparently acting as  some  sort of antigen retrieval step), and
immunohistochemistry on GMA sections does not work if one uses PBS as
opposed to TBS.

                                  Any help/hints gratefully received.
                                       Cheers
                                             Richard  Edwards
                                               University of
Leicester.
                                                    U.K.



------------------------------

Message: 16
Date: Wed, 04 Jun 2008 12:06:16 +0000
From: anitathorn@comcast.net 
Subject: RE: [Histonet] Creative Waste Solutions
To: "Laurie Colbert" ,	"Angela
	Bitting" ,
	
Message-ID:
	
<060420081206.14063.4846853800058ACF000036EF2200750330029D01089B0E9B07020E@c
omcast.net>
	

I work in a small volume lab and find that both the formalin and
alcohol recycling systems are perfect for our needs.  Easy to use and
very economical.
 -------------- Original message ----------------------
From: "Laurie Colbert" 
> Angie,
> 
> We use their formalin recycler, and we love it.  It is very easy to
use.
> 
> Laurie Colbert
> Huntington Hospital
> Pasadena, CA
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu 
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Angela
> Bitting
> Sent: Monday, June 02, 2008 4:24 AM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Creative Waste Solutions
> 
> Would users of recycling products from this company share feedback
with
> me?
> 
> Thanks,
> Angie
> 
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916 
>  
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
> 
> 
> 
> 
> IMPORTANT WARNING: The information in this message (and the
documents
> attached to it, if any) is confidential and may be legally
privileged.
> It is intended solely for the addressee. Access to this message by
> anyone else is unauthorized. If you are not the intended recipient,
any
> disclosure, copying, distribution or any action taken, or omitted to
be
> taken, in reliance on it is prohibited and may be unlawful. If you
have
> received this message in error, please delete all electronic copies
of
> this message (and the documents attached to it, if any), destroy any
> hard copies you may have created and notify me immediately by
replying
> to this email. Thank you.
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 17
Date: Wed, 4 Jun 2008 08:09:56 -0400
From: "Tom McNemar" 
Subject: RE: [Histonet] ML
T working histology?
To:
,	
Message-ID:
	<51D5D78FBEDAEA4FBCCD9A9D44211DC528F57A@lmhsmail.lmhealth.org>
Content-Type: text/plain;	charset="iso-8859-1"

I've had 3 or 4 MLT's over the years.  They were trained to do every
thing the HT's do.  They were hired with the stipulation that they got
their HT certification as well as having their MLT certification but
only because it was a requirement for all of us.  They have worked out
well.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org 
www.LMHealth.org 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie
Jenkins
Sent: Wednesday, May 28, 2008 1:51 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] MLT working histology?


Can an MLT work in histology? She would be embedding, cutting,
staining, 
cover slipping, labeling, filing, requisitioning and setting up the 
gross. If possible we could have her trained to gross in anything that

would not require "cutting", like GI biopsies.

Jennie Jenkins, PA(ASCP)
Southside Community Hospital
800 Oak Street
Farmville, Virginia 23901



==========================3D========================
This e-mail message (and attachments) may contain
information that is confidential to Southside
Community Hospital.  If you are not the intended
recipient you cannot use, distribute or copy the
message or attachments.  In such a case, please
notify the sender by return e-mail immediately and
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Opinions, conclusions and other information in
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------------------------------

Message: 18
Date: Wed, 4 Jun 2008 05:12:24 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] xylene grades
To: Merced Leiker ,
	histonet@lists.utsouthwestern.edu 
Message-ID: <472664.60493.qm@web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

The xylene "grade" (if such a thing exists!) is of a lesser concern
than assuring a complete dewaxing of the section. You have to completely
eliminate all the paraffin.
  Using distilled water to later immerse the sections in a buffer (that
contains salts) is of no relevance, because you are going to "salinize"
the sections any way.
  I think that your problem is with your Ab supplier, or the lot. CD117
is a "tricky" one.
  I stopped having problems when I switched to DAKO Mo Ab and HIER at
pH6
  René J.

Merced Leiker  wrote:
  Would the grade of xylenes used for deparaffinizing make a difference
for 
immunostaining of certain (picky) epitopes? We are using Certified
A.C.S. 
xylenes.

What about rinsing in tap water vs. distilled water during
rehydration?

The reason I'm asking is that we are having issues staining for c-kit
(KIT, 
CD117) on FFPE tissue. We've tried all different antigen retrievals, 
including no retrieval, and still aren't seeing any stain.

Any help would be greatly appreciated. Thanks!


Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


       

------------------------------

Message: 19
Date: Wed, 4 Jun 2008 08:20:19 -
0400
From: "Houston, Ronald" 
Subject: RE: [Histonet] MLT working histology?
To: "Tom McNemar" ,
	,	
Message-ID:
	
<979FF5962E234F45B06CF0DB7C1AABB20FEC203C@chi2k3ms01.columbuschildrens.net>
	
Content-Type: text/plain;	charset="us-ascii"

Why couldn't she? I have an MLT (taking her certification this year)
and
she is far superior to many of the "old timers" who claim to be
histotechs but have absolutely no theoretical knowledge and are thus
hopeless when it comes to troubleshooting. This profession needs to
take
the bull by the horns and change the outlook of histotechnology - it
is
a biomedical scientific profession and the training and examinations
should reflect this. It is already lagging far behind MT's. The HT
examination is a joke, and the QIHC certification is not much better.

Now let the flaming begin!!!!!!!!!!!


Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Ronald.Houston@NationwideChildrens.org 
www.NationwideChildrens.org 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Wednesday, June 04, 2008 8:10 AM
To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] MLT working histology?

I've had 3 or 4 MLT's over the years.  They were trained to do every
thing the HT's do.  They were hired with the stipulation that they got
their HT certification as well as having their MLT certification but
only because it was a requirement for all of us.  They have worked out
well.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org 
www.LMHealth.org 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie
Jenkins
Sent: Wednesday, May 28, 2008 1:51 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] MLT working histology?


Can an MLT work in histology? She would be embedding, cutting,
staining,

cover slipping, labeling, filing, requisitioning and setting up the 
gross. If possible we could have her trained to gross in anything that

would not require "cutting", like GI biopsies.

Jennie Jenkins, PA(ASCP)
Southside Community Hospital
800 Oak Street
Farmville, Virginia 23901



==========================3D========================
This e-mail message (and attachments) may contain
information that is confidential to Southside
Community Hospital.  If you are not the intended
recipient you cannot use, distribute or copy the
message or attachments.  In such a case, please
notify the sender by return e-mail immediately and
erase all copies of the message and attachments.
Opinions, conclusions and other information in
this message and attachments that do not relate to
the official business of Southside Community
Hospital are neither given nor endorsed by it.
==========================3D========================

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
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----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
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intended
 recipient), you are hereby notified that any review, use,
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reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.



------------------------------

Message: 20
Date: Wed, 4 Jun 2008 08:48:41 -0400
From: "Pam Barker" 
Subject: [Histonet] RELIA Special JOB Alert
To: 
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

Hi Histonetters!

I had one more position come in that I am pretty excited about.  I have
a
client in the Philadelphia area in need of a senior histotechnologist. 
They
are looking for someone who is ASCP certified with at least 5 years of
experience.  My client offers excellent compensation, benefits and a
great
place to work.  Dermatopathology experience is a plus.  If you are
interested please contact me.  I can be reached at relia1@earthlink.net
or
toll free at 866-607-3542.  If you know someone who might be
interested
please feel free to pass the information along to them as well.  

 

Incidentally if you happen to know an ASCP certified med tech with
microbiology experience I have a full time permanent 1st shift position
in
Cincinnati.  

 

Thanks and have a great day!! - Pam

 

Thank You!

 

Pam M. Barker

President

Relia

5703 Red Bug Lake Road #330

Winter Springs, FL 32708-4969

Phone: (407)657-2027

Cell:    (407)353-5070

FAX:    (407)678-2788

Toll Free: (866)607-3542

e-mail: relia1@earthlink.net 

http://home.earthlink.net/~relia1 

www.myspace.com/pamatrelia 

 



------------------------------

Message: 21
Date: Wed, 4 Jun 2008 13:55:24 +0100
From: "Ian Montgomery" 
Subject: [Histonet] ATPase.
To: 
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

            About to trial two techniques for the demonstration of
ATPase in
human skeletal muscle.

 

A Metachromatic Dye-ATPase Method for the Simultaneous Identification
of
Skeletal Muscle Fiber Types I, IIA, IIB and IIC.

Ogilvie,R.W. & Feeback,D.L. 1990. Stain Technol. 65. 231-242

 

Inhibition Reactivation Myofibrillar ATPase Technique for Demonstration
of
Three Fiber Types in a Single Cryostat Section.

Horak,V. & Matolin,S. 1990 Stain Technol. 65. 85-90

 

            Has anyone tried these techniques and do you have any
comments
on their efficacy? They both appear straightforward with the papers
giving
details of the method. But like everything there is always the wee
wrinkle
that's omitted that makes the difference. So, why re-invent the wheel
and
give myself even more grey hair when someone might have the answer.

Ian.

 

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 



------------------------------

Message: 22
Date: Wed, 4 Jun 2008 08:59:01 -0400
From: "Whitaker, Bonnie" 
Subject: RE: [Histonet] MLT working histology?
To: histonet@lists.utsouthwestern.edu 
Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F602624BF@msxc06.OSUMC.EDU>
Content-Type: text/plain; charset=us-ascii

Hi Everyone,

I wanted to chime in on this one.... 

I think that there are people with just about any background who will
make
good histotechs, and people with just about any background who will
make poor
histotechs.  It is very important for techs to understand the theory
behind
the procedures that they are doing, and this can be accomplished in
many
ways.  It generally will be better if someone has enough of a science
background to already understand some of the basic principles involved
in
histotechnology, but there will
 always be the exceptional person (on
both
sides of the curve).  

Rather than focusing on whether a person is an MLT, an MT, has a
biology
degree, etc., it would probably be better to focus on what kind of
drive that
person seems to have, and whether or not they are looking for a "job"
or for
a "career", and make it clear that they are expected to obtain their
registry
and undergo regular competency assessments that should do more than
"pay
lipservice" to the regulations.  (Of course I'm speaking for clinical
labs
that are CLIA and/or CAP.)

Bonnie Whitaker, MT(HEW), HT(ASCP)QIHC
Clinical Histology Manager
Ohio State University Medical Center
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210
614.293.5048
Bonnie.Whitaker@osumc.edu 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Houston,
Ronald
Sent: Wednesday, June 04, 2008 8:20 AM
To: Tom McNemar; jjenkins@sch-farmville.org;
histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] MLT working histology?

Why couldn't she? I have an MLT (taking her certification this year)
and she
is far superior to many of the "old timers" who claim to be histotechs
but
have absolutely no theoretical knowledge and are thus hopeless when it
comes
to troubleshooting. This profession needs to take the bull by the horns
and
change the outlook of histotechnology - it is a biomedical scientific
profession and the training and examinations should reflect this. It
is
already lagging far behind MT's. The HT examination is a joke, and the
QIHC
certification is not much better.

Now let the flaming begin!!!!!!!!!!!


Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Ronald.Houston@NationwideChildrens.org 
www.NationwideChildrens.org 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Wednesday, June 04, 2008 8:10 AM
To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] MLT working histology?

I've had 3 or 4 MLT's over the years.  They were trained to do every
thing
the HT's do.  They were hired with the stipulation that they got their
HT
certification as well as having their MLT certification but only
because it
was a requirement for all of us.  They have worked out well.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org 
www.LMHealth.org 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie
Jenkins
Sent: Wednesday, May 28, 2008 1:51 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] MLT working histology?


Can an MLT work in histology? She would be embedding, cutting,
staining,

cover slipping, labeling, filing, requisitioning and setting up the
gross. If
possible we could have her trained to gross in anything that would not
require "cutting", like GI biopsies.

Jennie Jenkins, PA(ASCP)
Southside Community Hospital
800 Oak Street
Farmville, Virginia 23901



==========================3D========================
This e-mail message (and attachments) may contain information that is
confidential to Southside Community Hospital.  If you are not the
intended
recipient you cannot use, distribute or copy the message or
attachments.  In
such a case, please notify the sender by return e-mail immediately and
erase
all copies of the message and attachments.
Opinions, conclusions and other information in this message and
attachments
that do not relate to the official business of Southside Community
Hospital
are neither given nor endorsed by it.
==========================3D========================

_______________________________________________
Histonet mailin
g list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

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----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the sole
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the intended recipient(s) and may contain confidential and privileged
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this information and to use the information only for authorized
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the intended recipient), you are hereby notified that any review, use,
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the contents of this e-mail is strictly prohibited. If you have
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and
destroy all copies of the original message. Thank you.

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------------------------------

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End of Histonet Digest, Vol 55, Issue 4
***************************************


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