RE: [Histonet] Rate of formalin penetration in human brain sections

From:"Marshall Terry Dr, Consultant Histopathologist"

Agree with Geoff entirely.

In particular you will not find it possible to define an end-point for
formalin fixation.


-----Original Message-----
[] On Behalf Of Geoff
Sent: 28 May 2008 15:19
Subject: Re: [Histonet] Rate of formalin penetration in human brain

Hi Karen:

    Formalin fixes tissue slowly, even very thin slices. This has been
known for many, many years. The work of Medwar and of John R. Baker
(Principles of Biological Technique, John Wiley and Sons, 1958) comes to
mind. Perhaps there are slight differences between kidney and brain but
my guess is that if there is a difference it is insignificant. Before
trying to design (how are you going to define fixation?) and perform
such investigations I suggest a trip to the library. I doubt if the
information you seek is on line, it is too old. However, do not confuse
"old" with "out dated" or "bad". Good luck.

Geoff wrote:
> Hi--
> One of our investigators is interested in the approximate rate of 
> fixation of human brain tissue, independent of any formaldehyde
diffusion effects.
> In other words, in a very small or very thin piece of human brain 
> tissue, what is the fixation rate? He found published data for rat 
> kidney, but would like to try to at least determine if the fixation 
> rate should be higher or lower in human brain tissue. He is looking 
> for data for room temperature, but any information would be greatly
> Thanks!
> Karen M Skish, MS, PA(ASCP)MT
> Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's

> Disease Center Cohn Research Building, Lab 441
> 1735 West Harrison Street
> Chicago IL 60612
> _______________________________________________
> Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583

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