Sebastian,
The following article uses a heated detergent solution to dewax and rehydrate sections. I have not as yet used it myself but I plan to try it at some time.

Lars Falkeholm, Crawford A. Grant, Anders Magnusson, and Eva Möller (2001) "Xylene-Free Method for Histological Preparation: A Multicentre Evaluation" LABORATORY INVESTIGATION Vol. 81, No. 9, p. 1213-1221.



Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebastián Vecchio
Sent: Tuesday, 20 May 2008 5:36 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave deparaffinization.


I have been trying to deparafinize section in the lab, but I cannot  get the right time and  power for the MW oven, does any one has a protocol for this . This method eliminates the uses  of xylol in the processing of staining sections. Thanks. Sebastian, Student of Histotechnology, EUTM, FMED, UDELAR. _______________________________________________
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