RE: [Histonet] MMA de-plasticizing

From:"Michelle McDonald"



Dear Michelle,

We de-plasticise MMP bone sections using two changes of 100% acetone for 10 monis each.

Thanks

Michelle

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich
Sent: Saturday, 31 May 2008 1:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MMA de-plasticizing


Is there anyone out there doing MMA on un-decalcified bone? I'm wondering if there is any way to de-plasticize without putting the slides in heated xylene--a potentially explosive situation which technically would require explosion proof oven, hood, clothing, etc. I know that the flash point of xylene is quite low (26.1C, I think, which is barely above room temperature). Is there a way around this safety issue?


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