RE: [Histonet] Immuno Controls

From:"Mickie Johnson"



Hi Histonetters,

I would like to add my 2 cents worth. When I started IHC it was by hand and
the tech was sure they had put primary Ab, etc on each slide. Then we went
automated with a Ventana ES. We learned the hard way that dispensers may or
may not dispense their contents and that this could vary from slide to slide
on the same run! This is when we began to put a control slide on each
positive patient slide for each antibody run on that patient and block.
Machines make mistakes too.

Thanks.

Best Regards,
 
Mickie
 
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX   509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com 
Email: mickie25@netzero.net
 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Friday, May 30, 2008 4:08 AM
To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Immuno Controls

I put control tissue on each slide I stain except the neg controls.

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel,
James F.
Sent: Thursday, May 29, 2008 2:11 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immuno Controls

Good Day Histonetters!  For immuno cases, how are you handling the control
tissue question?  Are you putting a piece of control tissue on each slide or
are
you using a control slide for each ab performed on a given day.  We are
using
Ventana'e Benchmark for the lion's share of our immunos.

Thanks!

James Happel
Massachusetts General Hospital


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