RE: [Histonet] Immuno Controls

From:Rene J Buesa

Ah the joys of using Ventana are endless!
René J.

Mark A Burton  wrote:  I'm sure this discussion must be in the archives but my understanding of how
the Ventana Benchmark works is that there is a plunger that presses on a
dispenser bottle to deliver the antibodies and reagents to the slide. If the
dispenser bottle is not primed or if there is some type of interference than
you would not get any antibody or reagent delivered to your slide. Without a
control on each slide, there is no way to know. Is this a legitimate
argument? or is the amount of time and tissue needed to accomplish this too

-----Original Message-----
[] On Behalf Of Rene J Buesa
Sent: Thursday, May 29, 2008 3:30 PM
To: Happel, James F.;
Subject: Re: [Histonet] Immuno Controls

I used the DAKO autostainer and the cases' sections were placed in slides
already containing a tissue control section in a way that both were
"stained" simultaneously.
I only used one single control for a whole series of cases when doing
Her2neu, or for research studies for our residents.
René J.

"Happel, James F." wrote:

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