RE: [Histonet] Formaldehyde + Acetic Acid

From:Rene J Buesa

And that is what is called EXPERIENCE = the intelligent use of mistakes made along our life!
  René J.

Lee & Peggy Wenk  wrote:
      Thanks for the nice words, but unfortunately, my answer was based on my own real life error.
  Some years ago, I was making alcoholic formalin for the tissue processor. I had already mixed the alcohol and water, then poured in the formaldehyde. And watched it turn cloudy milky white, to my utter amazement. My thoughts?
  - it's never done that before
  - I wonder if it will go away if I wait a couple of minutes (no)
  - I wonder if it will go away if I swirl the container and mix the solutions better (no)
  - I wonder what's wrong with the water today (no idea why I thought the water was the problem)
  Finally, I thought about looking at the labels on the solutions, and found that I had inadvertantly grabbed the 10% NBF instead of the 40% formaldehyde. Fortunately, I hadn't ruined any tissue, and all I had to do was make up new alcoholic formalin using the correct chemicals.
  However, that episode did teach me what can happen when I don't check each label before I use a chemical. And, I did get to see what happens when buffering salts and alcohol are mixed together. And this mistake then allowed me, many years later (now), to help Jennifer figure out her cloudiness problem.
  Peggy A. Wenk, HTL(ASCP)SLS
  William Beaumont Hospital
  Royal Oak, MI 48073

  From: Rene J Buesa [] 
Sent: Wednesday, May 21, 2008 9:29 AM
To: Jennifer Johnson;;; Elaine Smith
Subject: RE: [Histonet] Formaldehyde + Acetic Acid

  Peggy showed why she had been awarded "Histotech of the Year" with her very clever and insightful answer. 
  The salts dissolved in the NBF will precipitate with the alcohol. That is why the tissue processors have to be cleaned with hot hater (to eliminate the salts by dissolving them), or why some people (like me) used to use 10% formalin NOT buffered in the first 2 stations of the VIP (to avoid the salt precipitates).
  René J.

Jennifer Johnson  wrote:
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