Peggy showed why she had been awarded "Histotech of the Year" with her very clever and insightful answer. 
  The salts dissolved in the NBF will precipitate with the alcohol. That is why the tissue processors have to be cleaned with hot hater (to eliminate the salts by dissolving them), or why some people (like me) used to use 10% formalin NOT buffered in the first 2 stations of the VIP (to avoid the salt precipitates).
  René J.

Jennifer Johnson  wrote:
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