Yes, It was NBF. What started this whole mess was that my pathologist keeps a small holding tank of "somewhat clean?" NB formalin that he pours out of the biopsy containers when he grosses (He lived through the great depression). Then, he stores the basket from the processor in there while he grosses and at the end of the day, transfers it into the processor and starts it. One day, I had put a large breast specimen in FAA and he poured the FAA into the "holding tank" not knowing that it wasn't pure 10% NB formalin. (Yes, he is hard of smelling) It ruined all of the bloody specimens such as placenta (I assume because they are never fixed when they come down and the acetic acid lysed the red blood cells in it turning it to mush?) but everything else seemed to fair well. Now he is trying to figure out what the chemical reaction was that caused the problem. > From: lpwenk@sbcglobal.net> To: rjbuesa@yahoo.com; jmjohnson34@hotmail.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Formaldehyde + Acetic Acid> Date: Wed, 21 May 2008 05:52:31 -0400> > Just a thought - Could they have used neutral buffered formalin instead of> formaldehyde? The buffering salts could precipitate out in alcohol. > > > Lee & Peggy Wenk> -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa> Sent: Tuesday, May 20, 2008 8:58 AM> To: Jennifer Johnson; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Formaldehyde + Acetic Acid> > Not even that: FAA (formaldehyde + alcohol + acetic acid) + formalin do NOT> produce any milky solution. Tell you PT to check what s/he was mixing, that> had to be something else in that mixture.> René J.> > Jennifer Johnson wrote:> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
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