I think you're best bet is to try Biocare Medical's anti-Goat polymer
detection system -- which can be seen at:
http://www.biocaremed.com/promark.html. It is very easy to use and yeilds
very clean stains.
Date: Tue, 27 May 2008 10:55:59 +0800
From: "Min-Han Tan"
Subject: [Histonet] Biotin free detection of goat primary antibodies
I would like to enquire - I am using a goat primary antibody currently, and
my negative control (omitting primary antibody) is showing background
staining - this is likely a result of biotin.
I'd like to enquire if any one is familiar with biotin free systems that can
detect goat antibodies. A search of the Dako / Envision product line does
not yield any results. I am very reluctant to add an avidin-biotin blocking
step to what is already an overnight incubation.
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