Hello,
We've been doing some immunofluorescence in mouse embryos sections
(E9-10), and have recently encountered a problem involving very specific
immunofluorescent 'staining' in our negative controls. the staining
looks to be in specific cells in specific regions, and occurs in both
our no-primary and no-secondary controls. we are staining with a
rabbit anti-caspase, followed by an alexafluor-conjugated donkey
anti-rabbit. the background 'staining' if very bright and can be seen
with several different filters. has anyone else ever experienced
anything like this? we are stumped. I have never had this kind of
problem previously in other mouse tissues. if anyone has any insight, I
would greatly appreciate your input.
Thanks in advance,
Anna Beaudin
Division of Nutritional Sciences
Cornell University
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