[Histonet] osmium

From:"Nuernberger, Barb"

I'm asking this on behalf of my histologist who has been asked by a
pathologist to modify a protocol that she has from the AFIP manual.
When doing an osmium fixation on formalin fixed tissue, is the 8 hour
fixing time a minimum or can it sit overnight before continuing?

Thank you for any help.
Barb Nuernberger
Chief Toxicologist

Medical Examiner's Office
2100 Jefferson St. 
Jacksonville,FL 32206

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