My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin.
Thank you for your help!
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