There's always fluorescence--these authors used 100 um Vibratome
sections and found that the primary antibody may make a difference too.
Journal of Neuroscience Methods 170 (2008) 165–178
Associative image analysis: A method for automated quantification
of 3D multi-parameter images of brain tissue
Christopher S. Bjornsson, Gang Lin, Yousef Al-Kofahi, Arunachalam
Narayanaswamy, Karen L. Smith, William Shain, Badrinath Roysamb
Simple avidinylated HRP may be smaller than ABC complexes and penetrate
better. People have also used DMSO 1-10% in incubation solutions, and
ethanol extraction steps on cut thick sections, reported to improve
penetration (both in histonet archives).
UF Pharmacology & Therapeutics
Date: Tue, 27 May 2008 10:04:52 -0800
From: "Bob Nienhuis"
Subject: [Histonet] Thick section penetration for IHC
It has been suggested that the ABC detection system may not be the
optimal choice for IHC in thick brain sections (~100 microns).
Apparently, the large avidin-biotin complexes may not penetrate tissue
What would you suggest instead, LSAB, polymer or...?
UCLA / VA Medical Center