[Histonet] Thick section penetration for IHC

From:"Bob Nienhuis"

It has been suggested that the ABC detection system may not be the optimal
choice for IHC in thick brain sections (~100 microns). Apparently, the large
avidin-biotin complexes may not penetrate tissue too well.

What would you suggest instead, LSAB, polymer or...?

Bob Nienhuis
Neurobiology Research
UCLA / VA Medical Center
Los Angeles
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