[Histonet] Staining cultured cells with Giemsa

From:"Pixley, Sarah \(pixleysk\)"

Here is my staining procedure for monolayer cells in culture. As a
previous person mentioned, use your common sense to modify. But I worked
with it enough to know that the following steps worked for me.

Giemsa Stain:
Use Giemsa stain from Sigma:

?Rinse live cells 1x with PBS 1X (warranted to get rid of phenol red and
any serum)

Fix in cold 100% Methanol, 5-7 mins
Air Dry
Dilute Giemsa 1/20 with ddWater. ? Maybe it would be wise next time to
filter, Whatman#1
Stain 15-60 mins. For cultured cell monolayers, 15 was enough.
Rinse with ddWater. ~5-6 times.
Air dry
Can mount with aqueous mounting medium.

Sarah Pixley

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