Heidi,
I think it will depend on exactly what you are staining for. We routinely stain
3mm punch bx by fixing in Zamboni's for a few days, followed by cutting on a
sliding microtome w/ dry ice @ 50 microns, and then do free floating staining
for small nerve fibers. Our IHC's will only work with certain fixatives, PLP or
Zamboni's
Heather
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Friday, May 23, 2008 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 54, Issue 37
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. (no subject) (Jennifer Johnson)
2. RE: (no subject) (Connolly, Brett M)
3. RE: (no subject) (Pamela Marcum)
4. Validation (Sharon.Davis-Devine)
5. Skin biopsies (Heidi Miers)
6. Hematoxylin shortage (anitaibsc@aol.com)
7. Re: Leica Peloris (Joe Nocito)
8. RE: Hematoxylin shortage (dcojita@tampabay.rr.com)
9. looking for a service manual for the leica TP1050 (histocs)
10. Re: Microtome blades jammed in dispenser box (Matt Bancroft)
11. RE: Blades & Hematoxylin (Bartlett, Jeanine (CDC/CCID/NCZVED))
12. TN meeting Vendor Deadline Approaching! (Hofecker, Jennifer L)
13. 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH
7.4) fixative and immuno (michelle.schwab-macdonald@novartis.com)
14. RE: Blades & Hematoxylin (Horn, Hazel V)
15. Re: 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH
7.4) fixative and immuno (Rene J Buesa)
16. Histology Opening In Bar Harbor, Maine (Lesley Bechtold)
17. RE: Blades & Hematoxylin (Rosa Fields)
18. Mixing Monoclonal and Polyclonal Negative Reagents
(Kelly Colpitts)
19. Gi biopsies and polyps (Sharon Campbell)
20. RE: Blades & Hematoxylin (Greg Dobbin)
21. RE: Blades & Hematox Clarification (Greg Dobbin)
22. RE: Blades & Hematox Clarification (Blazek, Linda)
----------------------------------------------------------------------
Message: 1
Date: Thu, 22 May 2008 16:43:34 -0400
From: Jennifer Johnson
Subject: [Histonet] (no subject)
To: "histonet@lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/plain; charset="Windows-1252"
Dear Netters,
Last year I was planning a vacation to Albuquerque, NM and Sara Breeden gave me
the most excellent list of things to do! I figured I would try it again. Next
Wednesday I will be leaving for a two week vacation to Fiji, New Zealand,
Australia, and Hawaii. Any suggestions from you locals? Please reply off list
so as not to clog up the histonet. Thanks, Jennifer Johnson, HTL (ASCP)
_________________________________________________________________
Change the world with e-mail. Join the i'm Initiative from Microsoft.
http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld
------------------------------
Message: 2
Date: Thu, 22 May 2008 16:47:46 -0400
From: "Connolly, Brett M"
Subject: RE: [Histonet] (no subject)
To: "Jennifer Johnson" ,
Message-ID:
<63EA0607835FBA4689CEA9EA8B482692011BAC3F@usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"
I suggest you take the rest of us with you !!
Brett
Brett M. Connolly, Ph.D.
Research Fellow
MRL, Imaging Research
Merck & Co., Inc.
WP-44K
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803
e-mail. brett_connolly@merck.com
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer
Johnson
Sent: Thursday, May 22, 2008 4:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Dear Netters,
Last year I was planning a vacation to Albuquerque, NM and Sara Breeden
gave me the most excellent list of things to do! I figured I would try
it again. Next Wednesday I will be leaving for a two week vacation to
Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you
locals? Please reply off list so as not to clog up the histonet.
Thanks, Jennifer Johnson, HTL (ASCP)
_________________________________________________________________
Change the world with e-mail. Join the i'm Initiative from Microsoft.
http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWo
rld_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New
Jersey, USA 08889), and/or its affiliates (which may be known outside the United
States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu -
direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely for the
use of the individual or entity named on this message. If you are not the
intended recipient, and have received this message in error, please notify us
immediately by reply e-mail and then delete it from your system.
------------------------------
Message: 3
Date: Thu, 22 May 2008 17:05:25 -0400
From: Pamela Marcum
Subject: RE: [Histonet] (no subject)
To: "Connolly, Brett M" , "Jennifer Johnson"
,
Message-ID: <6.2.5.6.2.20080522170454.01cc51a0@vet.upenn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Absolutely Great Idea, Brett. She pays right??
Pam Marcum
At 04:47 PM 5/22/2008, Connolly, Brett M wrote:
>I suggest you take the rest of us with you !!
>
>Brett
>
>Brett M. Connolly, Ph.D.
>Research Fellow
>MRL, Imaging Research
>Merck & Co., Inc.
>WP-44K
>PO Box 4
>West Point, PA 19486
>PH 215-652-2501
>fax. 215-993-6803
>e-mail. brett_connolly@merck.com
>
>
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer
>Johnson
>Sent: Thursday, May 22, 2008 4:44 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] (no subject)
>
>
>Dear Netters,
>
>Last year I was planning a vacation to Albuquerque, NM and Sara Breeden
>gave me the most excellent list of things to do! I figured I would try
>it again. Next Wednesday I will be leaving for a two week vacation to
>Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you
>locals? Please reply off list so as not to clog up the histonet.
>Thanks, Jennifer Johnson, HTL (ASCP)
>_________________________________________________________________
>Change the world with e-mail. Join the i'm Initiative from Microsoft.
>http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWo
>rld_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>Notice: This e-mail message, together with any attachments,
>contains information of Merck & Co., Inc. (One Merck Drive,
>Whitehouse Station, New Jersey, USA 08889), and/or its affiliates
>(which may be known outside the United States as Merck Frosst, Merck
>Sharp & Dohme or MSD and in Japan, as Banyu - direct contact
>information for affiliates is available at
>http://www.merck.com/contact/contacts.html) that may be
>confidential, proprietary copyrighted and/or legally privileged. It
>is intended solely for the use of the individual or entity named on
>this message. If you are not the intended recipient, and have
>received this message in error, please notify us immediately by
>reply e-mail and then delete it from your system.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Best Regards,
Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr. CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348
Phone - 610-925-6278
Fax - 610-925-8120
E-mail - pmarcum@vet.upenn.edu
------------------------------
Message: 4
Date: Thu, 22 May 2008 16:13:37 -0500
From: "Sharon.Davis-Devine"
Subject: [Histonet] Validation
To:
Message-ID:
<44780C571F28624DBB446DE55C4D733A021E09A6@EXCHANGEBE1.carle.com>
Content-Type: text/plain; charset="us-ascii"
We are currently in the process of switching from a Dako immuno stainer
to the Ventana system. We need to do a validation for the new system but
need ideas from everyone out there who has done a validation to give us
some pointers on how to accomplish this. Any information is greatly
appreciated. Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology Supervisor
Carle Clinic
602 West University
Urbana, Illinois 61801
Phone: 217-383-3572
Email: sharon.davis-devine@carle.com
------------------------------
Message: 5
Date: Thu, 22 May 2008 15:27:01 -0700
From: Heidi Miers
Subject: [Histonet] Skin biopsies
To: histonet@lists.utsouthwestern.edu
Message-ID: <4835F335.2020509@nau.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Does anyone have any advice on tissue processing times for 0.5 to 1 mm
thick skin biopsies for IHC?
Thanks to all in advance,
Heidi
------------------------------
Message: 6
Date: Thu, 22 May 2008 18:51:10 -0400
From: anitaibsc@aol.com
Subject: [Histonet] Hematoxylin shortage
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CA8A757697861D-8C4-8D3@webmail-nd19.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Hello Netteres,
The information?I provided was to assist with a specific product and was not
intended as a mass, commercial advertisement.
IBSC makes variety of IHC products not just Hematoxylin.
Anita
------------------------------
Message: 7
Date: Thu, 22 May 2008 18:51:41 -0500
From: "Joe Nocito"
Subject: Re: [Histonet] Leica Peloris
To: "Laurie Colbert" , "Mike
Pence" , "Salcedo, Rudy" ,
Message-ID: <00c001c8bc66$c8d0c3b0$0302a8c0@yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
we just purchased two, with a third on the way. We took off the xylene and
the tissue has processed better. We had to monkey around with our fatty
specimens a little. We run a 4 hour and an 8 hour xylene-free program. Next
week, we try the 1.5 hour xylene free on real specimens. We are trying to
process throughout the day. So far, I like them. But, need to see the
response time if a repair needs to be made. This company has not had a good
track record in the past.
JTT
----- Original Message -----
From: "Laurie Colbert"
To: "Mike Pence" ; "Salcedo, Rudy" ;
Sent: Thursday, May 22, 2008 1:16 PM
Subject: RE: [Histonet] Leica Peloris
Do you use xylene on the Peloris?
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Thursday, May 22, 2008 9:19 AM
To: Salcedo, Rudy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Leica Peloris
I just purchased a Peloris about 3 months ago. Love it. I can process
all thru the day and it has made a huge impact on turn around times. We
cut fewer blocks thru out the day and I think it processes fatty
specimens better. I have a few small issues, but I have been very happy
with the processor.
Mike
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo,
Rudy
Sent: Thursday, May 22, 2008 10:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Peloris
Histonet,
Any labs out there using the Leica Peloris Processor, if so please send
me some feed back on you experience with the Peloris. Thanks in advance.
Rudy Salcedo
Houston, Texas
NOTICE: Our e-mail address at Methodist has changed to tmhs.org
Methodist. Leading Medicine.
Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to
Work For" in 2008 Named by U.S. News & World Report as one of "America's
Best Hospitals" Designated as a Magnet hospital for excellence in
nursing
***CONFIDENTIALITY NOTICE***
This e-mail is the property of The Methodist Hospital and/or its
relevant affiliates and may contain confidential and privileged material
for the sole use of the intended recipient(s). Any review, use,
distribution or disclosure by others is strictly prohibited. If you are
not the intended recipient (or authorized to receive for the recipient),
please contact the sender and delete all copies of the message. Thank
you.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 22 May 2008 21:15:06 -0400
From:
Subject: RE: [Histonet] Hematoxylin shortage
To: "'KELLY BOYD'" , "'histonet'"
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
This is fact. The hematoxylin trees were all wiped out due to past
hurricanes. The shortage is real.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KELLY BOYD
Sent: Thursday, May 22, 2008 9:51 AM
To: histonet
Subject: [Histonet] Hematoxylin shortage
Can someone please tell me what is up with all the talk about a hematoxylin
shortage? There is even a vendor telling their customers they need to stock
up. Is this fact or rumor?
Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
Tele (252)-830-6866
Cell (252)-943-9527
Fax (252)-830-0032
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Thu, 22 May 2008 18:54:15 -0700
From: "histocs"
Subject: [Histonet] looking for a service manual for the leica TP1050
To: "Histonet"
Message-ID: <000901c8bc77$e7f3bde0$6500a8c0@LHBLION>
Content-Type: text/plain; charset="Windows-1252"
Would anyone have a service manual for the TP1050. I would be happy to pay for
a copy or?
thanks
LeRoy Brown HT(ASCP)HTL
HCS
Everson, WA 98247
------------------------------
Message: 10
Date: Thu, 22 May 2008 19:16:37 -0700 (PDT)
From: Matt Bancroft
Subject: Re: [Histonet] Microtome blades jammed in dispenser box
To: Paula Pierce , Histonet
Message-ID: <611570.86726.qm@web63410.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I recently switched to the thermo blades, I think that they last longer and have
better sections.
I have not had any issues with these blades
Paula Pierce wrote:
I am having the same problem. The tab that grabs the hole in the blades to
push them forward tends to wear more than in the past and does not grab after a
few have been dispensed. I have taken a very fine pair of forceps and helped the
blade along and when that no loner works because the blade does not move at all
I took the box apart.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway Ave.
Moore, OK 73160
405-570-6679 cell
405-759-3953 lab
contact@excaliburpathology.com
www.excaliburpathology.com
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Fri, 23 May 2008 05:56:36 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)"
Subject: RE: [Histonet] Blades & Hematoxylin
To: "Breeden, Sara" ,
histonet@lists.utsouthwestern.edu
Message-ID:
<1CE1847DFEA0A647B1CCDE4108EA60A7F23A2F@LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
I wanted to give the SelecTech system a try and I even submitted slides
to Surgipath who said they would stain them and return them for our
review. After weeks passed I contacted Surgipath and was told they would
be coming soon. After a few more months I told them to forget it.
I was very disappointed that they were not responsive.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Thursday, May 22, 2008 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades & Hematoxylin
1. Blades: I use the Sakura/Feathers and I just give the container
a good "whack" (on the bottom) on the counter before I dispense the
first blade. They have no choice but to be compliant after that. even
think about our humidity level in NM at about 10%...
2. Hematoxylin: I have been reluctant because of my "position" as
president of the New Mexico Society for Histology to endorse products in
this venue, but the hematoxylin issue makes it necessary for me to break
my rule. I recently changed to the Surgipath "SelecTech" system and I
have to report rave reviews by my pathologists. The hematoxylin is good
for TWENTY-FIVE HUNDRED slides - the last slides being at least as good
as the first - and I've had no difficulty in getting a supply. Shelf
life is 2 years. Stain quality is superb. Cost is extremely reasonable
for the quality. My autostainer results had been dramatically variable
until I made the change to SelecTech and although I had tried it as an
economic move, it has proved to be that and more. The SelecTech system
gives me beautiful results. Bosses are happy as clams with the stain
now!
3. How happy IS a clam???
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Fri, 23 May 2008 09:02:11 -0500
From: "Hofecker, Jennifer L"
Subject: [Histonet] TN meeting Vendor Deadline Approaching!
To: "histonet"
Message-ID:
<898D946569A27444B65667A49C0740520175B4F9@mailbe06.mc.vanderbilt.edu>
Content-Type: text/plain; charset="us-ascii"
Happy Friday to everyone!
Just a brief reminder that the vendor registration deadline for the
Tennessee Society for Histotechnology Annual Meeting (June 19-21 in
Townsend, TN) is quickly approaching. If you have not yet registered and
are interested in exhibiting at our meeting, please contact me ASAP. All
registration materials and booth fees are due to me by May 30th.
If you need exhibiting information, I'll be glad to pass it along.
For everyone else, it's not too late to register to attend the meeting.
There are still hotel rooms available and a great variety of educational
offerings. Please visit the NSH website for complete program information
or feel free to contact me with questions.
Have a great (hopefully long) weekend!
Jennifer L. Hofecker HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
Nashville, TN
ph 615.343.0083
fax 615.343.7089
NSH Quality Control Committee Chairperson
TSH Secretary
TSH 2008 Exhibit Coordinator
------------------------------
Message: 13
Date: Fri, 23 May 2008 10:13:03 -0400
From: michelle.schwab-macdonald@novartis.com
Subject: [Histonet] 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M
PB, pH 7.4) fixative and immuno
To: histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
I am looking for anyone who might have experience with 4F1G (4%
Formaldehyde &1% Gluteraldehyde in 0.1M PBS, pH 7.4) and
immunohistochemistry staining. We are interested in fixing tissue in this
and then making both EM and paraffin blocks from the same sample. I was
interested in getting opinions on how different immuno markers, normally
run on FFPE tissue, might be affected by this fixation change. I realize
that it is probably marker dependent and that fixation is a huge factor in
immuno, but I was just interested in some opinions or any markers that you
might have tried this fixative with that will work.
Thanks in Advance!! :-)
Michelle Schwab-MacDonald HT(ASCP)
Novartis, Boston, MA
------------------------------
Message: 14
Date: Fri, 23 May 2008 09:16:22 -0500
From: "Horn, Hazel V"
Subject: RE: [Histonet] Blades & Hematoxylin
To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Breeden,
Sara" ,
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C7A@EMAIL.archildrens.org>
Content-Type: text/plain; charset="us-ascii"
Jeanine I am sorry you had this experience. My Surgipath rep came to my
lab and worked with us as we stained slides with the new stains to try
them out. They are excellent.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Friday, May 23, 2008 4:57 AM
To: Breeden, Sara; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Blades & Hematoxylin
I wanted to give the SelecTech system a try and I even submitted slides
to Surgipath who said they would stain them and return them for our
review. After weeks passed I contacted Surgipath and was told they would
be coming soon. After a few more months I told them to forget it.
I was very disappointed that they were not responsive.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Thursday, May 22, 2008 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades & Hematoxylin
1. Blades: I use the Sakura/Feathers and I just give the container
a good "whack" (on the bottom) on the counter before I dispense the
first blade. They have no choice but to be compliant after that. even
think about our humidity level in NM at about 10%...
2. Hematoxylin: I have been reluctant because of my "position" as
president of the New Mexico Society for Histology to endorse products in
this venue, but the hematoxylin issue makes it necessary for me to break
my rule. I recently changed to the Surgipath "SelecTech" system and I
have to report rave reviews by my pathologists. The hematoxylin is good
for TWENTY-FIVE HUNDRED slides - the last slides being at least as good
as the first - and I've had no difficulty in getting a supply. Shelf
life is 2 years. Stain quality is superb. Cost is extremely reasonable
for the quality. My autostainer results had been dramatically variable
until I made the change to SelecTech and although I had tried it as an
economic move, it has proved to be that and more. The SelecTech system
gives me beautiful results. Bosses are happy as clams with the stain
now!
3. How happy IS a clam???
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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intended recipient, or an employee or agent responsible for delivering this
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dissemination, distribution or copying of this communication is strictly
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Thank you.
------------------------------
Message: 15
Date: Fri, 23 May 2008 07:27:11 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] 4F1G (4% Formaldehyde & 1% Glutaraldehyde in
0.1 M PB, pH 7.4) fixative and immuno
To: michelle.schwab-macdonald@novartis.com,
histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID: <486224.7212.qm@web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Generally speaking you cannot get a good preservation/fixation for TEM if the
block is also processed for paraffin infiltration.
The reason being the thickness/size of the slice of tissue that HAS to be of
1-2 cubic mm to assure a good preservation for TEM.
Other than that you just will probably have to make a stronger HIER before the
IHC.
I would treat one slice of tissue for FFPE and another for TEM.
René J.
michelle.schwab-macdonald@novartis.com wrote:
I am looking for anyone who might have experience with 4F1G (4%
Formaldehyde &1% Gluteraldehyde in 0.1M PBS, pH 7.4) and
immunohistochemistry staining. We are interested in fixing tissue in this
and then making both EM and paraffin blocks from the same sample. I was
interested in getting opinions on how different immuno markers, normally
run on FFPE tissue, might be affected by this fixation change. I realize
that it is probably marker dependent and that fixation is a huge factor in
immuno, but I was just interested in some opinions or any markers that you
might have tried this fixative with that will work.
Thanks in Advance!! :-)
Michelle Schwab-MacDonald HT(ASCP)
Novartis, Boston, MA
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Fri, 23 May 2008 10:35:56 -0400
From: "Lesley Bechtold"
Subject: [Histonet] Histology Opening In Bar Harbor, Maine
To: "histonet@lists.utsouthwestern.edu"
Message-ID: <20080523103556689.00000001892@spikey>
Content-Type: text/plain; charset=us-ascii
There is a fulltime position in the Histology Service of The Jackson Laboratory
as a Histotechnologist II/III. Responsibilities include conduct of standard
histological protocols including embedding, sectioning and staining as well as
routine laboratory maintenance and administrative tasks. Minimum qualifications
include Associate's degree in a biological science and HT(ASCP) certification
plus 3-5 years of experience in histology OR a Bachelor's degree in a biological
field and a minimum 3 years of experience in histology. Experience in murine
histology and specialized techniques such as serial sectioning,
immunohistochemistry, plastic embedding and plastic sectioning is helpful. The
incumbent will have the opportunity to further their skills and knowledge.
Required computer skills include email, internet, word processing, spreadsheets
and familiarity with databases. Effective written and verbal communication
skills are essential. Successful applicants will demonstrate good interpersonal
skills and must have the ability and willingness to function effectively in a
team environment.
The Jackson Laboratory is one of the world's foremost centers for mammalian
genetics research. Located in Bar Harbor, Maine, the lab is adjacent to Acadia
National Park. Mountains, ocean, forests, lakes, and trails are all within
walking distance. If you are looking for a more natural environment, this could
be the opportunity you've been searching for.
Interested individuals should apply on-line on the internet at www.jax.org,
click on Careers at the top of the page. On Current Opportunities, click on
"Bar Harbor", type in "Histology" for a keyword and click on "search". This
will go directly to Job Listing 00001127. Please submit cover letter and resume
online as one document.
Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322
------------------------------
Message: 17
Date: Fri, 23 May 2008 09:49:18 -0500
From: "Rosa Fields"
Subject: RE: [Histonet] Blades & Hematoxylin
To: "Horn, Hazel V" , "Bartlett, Jeanine
\(CDC/CCID/NCZVED\)" , "Breeden, Sara"
,
Message-ID:
<2F2611250DCD6549AA3D96CE8AF1F0180109C3B6@giexchange.gidocs.net>
Content-Type: text/plain; charset="iso-8859-1"
Our Surgipath rep came to our lab also, the stain is excellent, I am very happy
with it also.
Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE 68503
402-465-4545
rfields@gidocs.net
The information contained in the message and the documents accompanying this
message contain information that is privileged and confidential and is intended
only for the use of the individual or entity named above. If the reader of this
message is not the intended recipient or the employee or agent responsible for
delivering it to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication, other than its
return to the sender, is strictly prohibited.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, May 23, 2008 9:16 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Breeden, Sara;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Blades & Hematoxylin
Jeanine I am sorry you had this experience. My Surgipath rep came to my
lab and worked with us as we stained slides with the new stains to try
them out. They are excellent.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Friday, May 23, 2008 4:57 AM
To: Breeden, Sara; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Blades & Hematoxylin
I wanted to give the SelecTech system a try and I even submitted slides
to Surgipath who said they would stain them and return them for our
review. After weeks passed I contacted Surgipath and was told they would
be coming soon. After a few more months I told them to forget it.
I was very disappointed that they were not responsive.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Thursday, May 22, 2008 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades & Hematoxylin
1. Blades: I use the Sakura/Feathers and I just give the container
a good "whack" (on the bottom) on the counter before I dispense the
first blade. They have no choice but to be compliant after that. even
think about our humidity level in NM at about 10%...
2. Hematoxylin: I have been reluctant because of my "position" as
president of the New Mexico Society for Histology to endorse products in
this venue, but the hematoxylin issue makes it necessary for me to break
my rule. I recently changed to the Surgipath "SelecTech" system and I
have to report rave reviews by my pathologists. The hematoxylin is good
for TWENTY-FIVE HUNDRED slides - the last slides being at least as good
as the first - and I've had no difficulty in getting a supply. Shelf
life is 2 years. Stain quality is superb. Cost is extremely reasonable
for the quality. My autostainer results had been dramatically variable
until I made the change to SelecTech and although I had tried it as an
economic move, it has proved to be that and more. The SelecTech system
gives me beautiful results. Bosses are happy as clams with the stain
now!
3. How happy IS a clam???
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify
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------------------------------
Message: 18
Date: Fri, 23 May 2008 10:52:39 -0400
From: Kelly Colpitts
Subject: [Histonet] Mixing Monoclonal and Polyclonal Negative Reagents
To:
Message-ID:
Content-Type: text/plain; charset="Windows-1252"
To Whom It May Concern,I had posted this question earlier in the week but I
realize that it was difficult to read so here it is again.
Recently our lab was told that we would combine our pre-made Monoclonal Negative
Antibody and our pre-made Polyclonal Negative Antibody in the same dispenser to
run as the negative reagent on all cases. Would it be ok to mix a Mouse
Monoclonal and a Rabbit Polyclonal or is the difference in species going to
cause a problem?
Thanks for your help!
_________________________________________________________________
Change the world with e-mail. Join the i'm Initiative from Microsoft.
http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld
------------------------------
Message: 19
Date: Fri, 23 May 2008 11:14:31 -0400
From: "Sharon Campbell"
Subject: [Histonet] Gi biopsies and polyps
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Hello Histoworld,
My lab is going to be doing many GI biopsies (new clients). Does anyone
have a good short processing schedule for GI biopsies? Also, can GI
polyps be on the same processing schedule?
Thank you in advance,
Sharon Campbell
------------------------------
Message: 20
Date: Fri, 23 May 2008 13:18:03 -0300
From: "Greg Dobbin"
Subject: RE: [Histonet] Blades & Hematoxylin
To: ,,
Message-ID:
Content-Type: text/plain; charset=US-ASCII
My experience with sectTech reagents is identical to that described by
Jeanine (below). Big time saver when I am not troubleshooting H&E
quality every other week!!
Greg
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
>>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008
6:56:36 AM >>>
I wanted to give the SelecTech system a try and I even submitted
slides
to Surgipath who said they would stain them and return them for our
review. After weeks passed I contacted Surgipath and was told they
would
be coming soon. After a few more months I told them to forget it.
I was very disappointed that they were not responsive.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Breeden,
Sara
Sent: Thursday, May 22, 2008 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades & Hematoxylin
1. Blades: I use the Sakura/Feathers and I just give the container
a good "whack" (on the bottom) on the counter before I dispense the
first blade. They have no choice but to be compliant after that. even
think about our humidity level in NM at about 10%...
2. Hematoxylin: I have been reluctant because of my "position" as
president of the New Mexico Society for Histology to endorse products
in
this venue, but the hematoxylin issue makes it necessary for me to
break
my rule. I recently changed to the Surgipath "SelecTech" system and I
have to report rave reviews by my pathologists. The hematoxylin is
good
for TWENTY-FIVE HUNDRED slides - the last slides being at least as
good
as the first - and I've had no difficulty in getting a supply. Shelf
life is 2 years. Stain quality is superb. Cost is extremely reasonable
for the quality. My autostainer results had been dramatically variable
until I made the change to SelecTech and although I had tried it as an
economic move, it has proved to be that and more. The SelecTech system
gives me beautiful results. Bosses are happy as clams with the stain
now!
3. How happy IS a clam???
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged
information intended for a specific individual or organization. If you have
received this communication in error, please notify the sender immediately. If
you are not the intended recipient, you are not authorized to use, disclose,
distribute, copy, print or rely on this email, and should promptly delete this
email from your entire computer system.
D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des renseignements
confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si
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l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous
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courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de
votre syst?me informatique.
-------------------------
------------------------------
Message: 21
Date: Fri, 23 May 2008 13:20:27 -0300
From: "Greg Dobbin"
Subject: RE: [Histonet] Blades & Hematox Clarification
To: ,,
Message-ID:
Content-Type: text/plain; charset=US-ASCII
I should have said my experience was the same as Sally's (ie very
positive).
Cheers.
Greg
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
>>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008
6:56:36 AM >>>
I wanted to give the SelecTech system a try and I even submitted
slides
to Surgipath who said they would stain them and return them for our
review. After weeks passed I contacted Surgipath and was told they
would
be coming soon. After a few more months I told them to forget it.
I was very disappointed that they were not responsive.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Breeden,
Sara
Sent: Thursday, May 22, 2008 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades & Hematoxylin
1. Blades: I use the Sakura/Feathers and I just give the container
a good "whack" (on the bottom) on the counter before I dispense the
first blade. They have no choice but to be compliant after that. even
think about our humidity level in NM at about 10%...
2. Hematoxylin: I have been reluctant because of my "position" as
president of the New Mexico Society for Histology to endorse products
in
this venue, but the hematoxylin issue makes it necessary for me to
break
my rule. I recently changed to the Surgipath "SelecTech" system and I
have to report rave reviews by my pathologists. The hematoxylin is
good
for TWENTY-FIVE HUNDRED slides - the last slides being at least as
good
as the first - and I've had no difficulty in getting a supply. Shelf
life is 2 years. Stain quality is superb. Cost is extremely reasonable
for the quality. My autostainer results had been dramatically variable
until I made the change to SelecTech and although I had tried it as an
economic move, it has proved to be that and more. The SelecTech system
gives me beautiful results. Bosses are happy as clams with the stain
now!
3. How happy IS a clam???
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged
information intended for a specific individual or organization. If you have
received this communication in error, please notify the sender immediately. If
you are not the intended recipient, you are not authorized to use, disclose,
distribute, copy, print or rely on this email, and should promptly delete this
email from your entire computer system.
D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des renseignements
confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si
vous avez re?u la pr?sente communication par erreur, veuillez en informer
l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous
n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce
courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de
votre syst?me informatique.
-------------------------
------------------------------
Message: 22
Date: Fri, 23 May 2008 12:36:59 -0400
From: "Blazek, Linda"
Subject: RE: [Histonet] Blades & Hematox Clarification
To: "Greg Dobbin" , ,
,
Message-ID:
<1F937FB30BDB7C4A9F39F83FEA8D379F9F5501@bruexchange1.digestivespecialists.com>
Content-Type: text/plain; charset="us-ascii"
Mine experience with SelecTech has also been great. They are a
wonderful stain and the rep helped us get all of the protocols set up.
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek@digestivespecialists.com
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg
Dobbin
Sent: Friday, May 23, 2008 12:20 PM
To: jqb7@cdc.gov; histonet@lists.utsouthwestern.edu;
sbreeden@nmda.nmsu.edu
Subject: RE: [Histonet] Blades & Hematox Clarification
I should have said my experience was the same as Sally's (ie very
positive).
Cheers.
Greg
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
>>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008
6:56:36 AM >>>
I wanted to give the SelecTech system a try and I even submitted
slides
to Surgipath who said they would stain them and return them for our
review. After weeks passed I contacted Surgipath and was told they
would
be coming soon. After a few more months I told them to forget it.
I was very disappointed that they were not responsive.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Breeden,
Sara
Sent: Thursday, May 22, 2008 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades & Hematoxylin
1. Blades: I use the Sakura/Feathers and I just give the container
a good "whack" (on the bottom) on the counter before I dispense the
first blade. They have no choice but to be compliant after that. even
think about our humidity level in NM at about 10%...
2. Hematoxylin: I have been reluctant because of my "position" as
president of the New Mexico Society for Histology to endorse products
in
this venue, but the hematoxylin issue makes it necessary for me to
break
my rule. I recently changed to the Surgipath "SelecTech" system and I
have to report rave reviews by my pathologists. The hematoxylin is
good
for TWENTY-FIVE HUNDRED slides - the last slides being at least as
good
as the first - and I've had no difficulty in getting a supply. Shelf
life is 2 years. Stain quality is superb. Cost is extremely reasonable
for the quality. My autostainer results had been dramatically variable
until I made the change to SelecTech and although I had tried it as an
economic move, it has proved to be that and more. The SelecTech system
gives me beautiful results. Bosses are happy as clams with the stain
now!
3. How happy IS a clam???
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or
privileged information intended for a specific individual or
organization. If you have received this communication in error, please
notify the sender immediately. If you are not the intended recipient,
you are not authorized to use, disclose, distribute, copy, print or rely
on this email, and should promptly delete this email from your entire
computer system.
D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des
renseignements confidentiels ? l'intention d'une personne ou d'un
organisme particulier. Si vous avez re?u la pr?sente communication par
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pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser,
divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous
en servir, et vous devriez l'effacer imm?diatement de votre syst?me
informatique.
-------------------------
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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