Several things to check if frozen section slides appear inadequately blued:
The hematoxylin needs to be fresh, filtered daily, and covered when not in use.
Don't use a regressive (acid-differentiated) hematoxylin for frozen sections.
Slosh the slide around in the hematoxylin until it's thoroughly wetted
and doesn't "bead" on the slide. Stain for long enough (usually about
Lithium carbonate is the simplest and strongest bluing agent for
frozen sections, though the others work also. Put dry lithium
carbonate into water in a bottle (not glass-stoppered) until a fine
white sediment appears on the bottom - that means that the LiCO3,
which is sparingly soluble in water, is saturated. Pour some off into
a Coplin jar and top off the bottle with more water (tap water will
usually do). Lithium carbonate has some medical uses, but it is not a
controlled substance. When I'm on the road, I carry a supply with me
to use in labs where the frozen section bluing is inadequate.
Be sure to look at the slide yourself!
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