I wanted to say something about the negative controls "The positive control slide or patient test slides, if these
slides contain tissue elements that should not react with the antibody."
This means to me and my facility that there is no need for negative controls- that your negative is making sure your positive control is staining correctly and not staining what it isn't supposed to and you have an accurate control.
Jen
MCRMC
Mi
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Saturday, June 07, 2008 12:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 55, Issue 13
Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Looking for Work (Steven Coakley)
2. RE: Sally's Friday Fume (Joyce Cline)
3. Veterinary Histologist Certification Eligibility (Breeden, Sara)
4. Re: Sally's Friday Fume (Shelly Christenson)
5. Job Opening Columbus, OH (Gullifer, Dawn)
6. RE: Sally's Friday Fume (Gessford, Mary)
7. unsubscribe (Terry Costello)
8. Peroxidase blocking in frozen sections (JMyers1@aol.com)
9. RE: Sally's Friday Fume (Liz Chlipala)
10. RE: Veterinary Histologist Certification Eligibility
(Della Speranza, Vinnie)
11. SLide Holder (Behnaz Sohrab)
12. IHC neg. controls (Friday rant) (Patti Loykasek)
13. Re: IHC neg. controls (Friday rant) (Patti Loykasek)
14. Re: IHC neg. controls (Friday rant) (Larry Woody)
15. Re: IHC neg. controls (Friday rant) (Rene J Buesa)
16. RE:Peroxidase blocking in frozen sections (JR R)
17. Re: IHC neg. controls (Friday rant) (pruegg@ihctech.net)
18. pAKT (Andrea Hooper)
19. Re: pAKT (pruegg@ihctech.net)
20. Re: Sally's Friday Fume (Janet Maass)
21. Disposing of used paraffin from the tissue processor
(Scott, Allison D)
22. Re: Disposing of used paraffin from the tissue processor
(Rene J Buesa)
23. Re: NSH Histology Task Analysis and StandardsofPerformance
Competen cy (Linda)
----------------------------------------------------------------------
Message: 1
Date: Fri, 6 Jun 2008 10:31:58 -0700 (PDT)
From: Steven Coakley
Subject: [Histonet] Looking for Work
To: Histonet@lists.utsouthwestern.edu
Message-ID: <193638.55208.qm@web38201.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I reside in South Central WI. Looking for work in this area, No. WI, No.Mich. VT, or NH.
Thanks everyone and have a "grand" weekend.
Steve
------------------------------
Message: 2
Date: Fri, 6 Jun 2008 13:39:12 -0400
From: "Joyce Cline"
Subject: [Histonet] RE: Sally's Friday Fume
To: "Histonet"
Message-ID: <74D382FF830A469BA98DB51E36C9FBF5@wchsys.org>
Content-Type: text/plain; charset="US-ASCII"
I received my HT while I worked in research, under a veterinarian
pathologist.
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Message: 3
Date: Fri, 6 Jun 2008 12:03:39 -0600
From: "Breeden, Sara"
Subject: [Histonet] Veterinary Histologist Certification Eligibility
To:
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B017E6370@nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
That's a mouthful! Anyway - thank you to those who responded to me. I
searched out a board-certified pathologist (non-veterinary) who works in
our building (we share a State of NM building with OMI and the State
Laboratory) and he was as astounded as I. I appreciate the feedback
about writing a letter to BOR requesting a "waiver" for a veterinary
situation and I will try that when the time comes. Our lab, in
anticipation of our new building (2010) is operating under conditional
AALVD certification but certainly not under CAP/JCHAO. I'm thinking of
asking for a definition of "medical scientist" from the ASCP. The saga
continues, but "where there's a will, there's a way". One would think
anyone who is interested in becoming a certified histologist would
receive the maximum amount of encouragement, from the employer AND the
certifying agency. Perhaps it's time for a review of the requirements
or at least of the definition of an employer's validity to provide
appropriate training, hmmm??? Mr. Della Speranza, do you have a take on
this?
It is not my goal here to fluster, annoy, or pester. It's just that I'm
beginning to see a certain amount of reluctance on the part of those
institutions whose interest should be to encourage the growth of our
profession. Now that my aim is to interest local mid-high and high
school forensic science students in a detour to the Paraffin Side, I
have a path littered with roadblocks. If our profession had the same PR
firm that the anti-tobacco industry had, we'd be up to our earlobes in
histotechs (did that come out right?).
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
------------------------------
Message: 4
Date: Fri, 06 Jun 2008 13:14:12 -0500
From: "Shelly Christenson"
Subject: Re: [Histonet] Sally's Friday Fume
To:
Message-ID: <48493823.EF61.003F.0@vet.k-state.edu>
Content-Type: text/plain; charset=US-ASCII
I work in a veterinary diagnostic lab and got my HT certification in 1993 without any problems and they accepted our veterinary pathologist signature. Two other techs got certified later and no problems there either.
Shelly Christenson HT(ASCP)
Kansas State University
Veterinary Diagnostic Lab
Manhattan, Kansas 66506
>>> "Breeden, Sara" 6/6/2008 11:28 AM >>>
Okay, I finally have something to Fume about. I am beginning to train a
person to become a certified histologist. She is a certified veterinary
tech with an Associates Degree and the appropriate number of
chemistry/biology hours. I inquired of ASCP about her eligibility (after
we finish the training period) to take the HT exam. According to ASCP,
as a vet diagnostic lab, we do not meet the ASCP requirements for
eligibility because we are not accredited by CAP, JCAHO/AABB and do not
have a board-certified (read "human") pathologist/medical scientist
(hmmm? Could one of our veterinary pathologists fulfill the description
of "medical scientist"??). Surely there are other persons working in a
veterinary pathology lab that would be interested in certification, one
would think. What are they to do? This frosts my petunias and I'd like
to know if anyone has any experience or input on this. But - hey! - some
of my fluids/solutions in gallon/liter containers seem to have been
improved so they don't drip, so maybe someone out there IS listening
(that was my first Friday Hour of Fuming subject). Hasta lumbago.
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Fri, 6 Jun 2008 14:31:31 -0400
From: "Gullifer, Dawn"
Subject: [Histonet] Job Opening Columbus, OH
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=iso-8859-1
All Interested-
We have a job opening for a Anatomic Pathology Technologist at OSU Histology
Lab, LLC. We are located in Columbus Ohio and are affiliated with The Ohio
State University Medical Center. The duties include accessioning, gross
examination of biopsy specimens including dictation, preparing tissue for
processing and all other normal laboratory duties (inventory, recording,
supplies etc....). Experience Requirements are a 2 yr. associates degree in
science related field or minimum 24 semester hours (36 quarter hours) of
biology, chemistry, physics, and math. The Job Qualifications include
training or experience as a tissue prosector desired; certification or
experience as a histology technologist desired; and certification, training
or experience as an anatomic pathology technician desired. For additional
information and for qualifying candidates, please contact me at
dawn.gullifer@osumc.edu.
Dawn Gullifer BS, HT (ASCP)
Laboratory Supervisor
OSU Histology Lab, LLC
614-293-0358 office
614-293-0345 lab
dawn.gullifer@osumc.edu
This e-mail, including attachments, may include confidential and/or
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------------------------------
Message: 6
Date: Fri, 6 Jun 2008 14:33:28 -0400
From: "Gessford, Mary"
Subject: RE: [Histonet] Sally's Friday Fume
To: "Breeden, Sara" ,
Message-ID:
Content-Type: text/plain; charset="us-ascii"
I don't believe the rules have changed. Unless they changed in the last
few weeks.
I just sent verification for one of my techs to take the HTL in the last
2 months and had no problems. We are a large Pharma, our lab is all Vet
Path.
There are at least 21 Vet schools in the US and a State Diagnostic lab
in every State in the country.
My HT goes back to 1982 and my HTL and IHC are from the 2003 and 2005.
The VIR group of the NSH is a large group of Registered techs.
We have always been covered under the same ASCP. There is no
distinction between human and animal. In fact, when grading the
practical (that was discontinued) in the last 10 years or so the ASCP
always had a vet path and histo tech from VIR on the grading committee.
I believe Liz was a grader.
You should call ASCP again and set them strait, maybe I will!
Mary Gessford BA,HT,HTL(ASCP)IHC
Scientist II Supervisor
Anatomic Pathology
Schering-Plough
Summit, NJ
908-473-4358
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Friday, June 06, 2008 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sally's Friday Fume
Okay, I finally have something to Fume about. I am beginning to train a
person to become a certified histologist. She is a certified veterinary
tech with an Associates Degree and the appropriate number of
chemistry/biology hours. I inquired of ASCP about her eligibility (after
we finish the training period) to take the HT exam. According to ASCP,
as a vet diagnostic lab, we do not meet the ASCP requirements for
eligibility because we are not accredited by CAP, JCAHO/AABB and do not
have a board-certified (read "human") pathologist/medical scientist
(hmmm? Could one of our veterinary pathologists fulfill the description
of "medical scientist"??). Surely there are other persons working in a
veterinary pathology lab that would be interested in certification, one
would think. What are they to do? This frosts my petunias and I'd like
to know if anyone has any experience or input on this. But - hey! - some
of my fluids/solutions in gallon/liter containers seem to have been
improved so they don't drip, so maybe someone out there IS listening
(that was my first Friday Hour of Fuming subject). Hasta lumbago.
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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intended recipient. If you are not the intended recipient,
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immediately and permanently delete.
------------------------------
Message: 7
Date: Fri, 6 Jun 2008 13:45:00 -0500
From: "Terry Costello"
Subject: [Histonet] unsubscribe
To:
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
Thanks.
Terry Costello
Senior Laboratory Animal Technologist
GTx, Inc 3rd Floor Van Vleet Bldg.
Memphis, TN 38163
900-523-9700 ext. 102
Fax: 901-523-9772
tcostello@gtxinc.com
This electronic message, including any attachments, is confidential and proprietary and is solely for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronic transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments.
------------------------------
Message: 8
Date: Fri, 6 Jun 2008 15:00:57 EDT
From: JMyers1@aol.com
Subject: [Histonet] Peroxidase blocking in frozen sections
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
'Netters:
I'd like to know what reagents/methods people are using to block for
endogenous peroxidase in frozen sections. Any feedback that you might be willing to
offer is greatly appreciated.
Joe
**************Get trade secrets for amazing burgers. Watch "Cooking with
Tyler Florence" on AOL Food.
(http://food.aol.com/tyler-florence?video=4?&NCID=aolfod00030000000002)
------------------------------
Message: 9
Date: Fri, 6 Jun 2008 13:05:30 -0600
From: "Liz Chlipala"
Subject: RE: [Histonet] Sally's Friday Fume
To: "Gessford, Mary" , "Breeden, Sara"
,
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Mary is right, I was a grader and also sat on the BOR for about 8 or so
years back in the late 80's and 90's. There was always representation
on both the Board of Registry Histotechnology Exam Committee and within
the pool of individuals who were slide graders from VIR, both veterinary
pathologists, HT's or HTL's that worked in research. It has always been
a very balanced group of individuals consisting of techs, veterinary
pathologists and MD pathologists. At least when I was invloved.
In my situation here, since this is my own lab and there is no medical
director or pathologist who could sign we needed to write a letter to
the BOR, that's what they told us to do at the time my tech sat for the
HTL and when the two of us took the IHC qualification, that was around
2002 or 2003 I think. I'm just guessing but I think that the individual
who told you this info was misinformed not unless things have changed.
Is there anyone out there who is on the current BOR Histotechnology exam
committee comment?
I just reviewed the HTL requirements on line an here is what it states,
it looks like experience in any histopathology laboratory will do (am I
reading this correctly?) so I think that the person you spoke to at ASCP
was misinformed.
To be eligible for this examination category, an applicant must satisfy
the requirements of at least one of the following routes:
Route 1: Baccalaureate degree from a regionally accredited
college/university with a combination of 30 semester hours (45 quarter
hours) of biology and chemistry AND successful completion of a NAACLS
accredited Histotechnician or Histotechnology program within the last 5
years; or
Route 2: Baccalaureate degree from a regionally accredited
college/university with a combination of 30 semester hours (45 quarter
hours) of biology and chemistry AND one year full time acceptable
experience in a histopathology laboratory in the U.S., Canada or a
CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the
last ten years. This year of experience must be under the supervision of
a pathologist (certified by the American Board of Pathology in Anatomic
Pathology) or an appropriately board certified medical scientist.
Clinical Laboratory Experience
To fulfill the experience requirement for the Histotechnologist
examination, you must have experience, within the last ten years, in the
following areas:
Fixation
Microtomy
Processing
Staining
Thanks
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz@premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Gessford, Mary
Sent: Friday, June 06, 2008 12:33 PM
To: Breeden, Sara; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Sally's Friday Fume
I don't believe the rules have changed. Unless they changed in the last
few weeks.
I just sent verification for one of my techs to take the HTL in the last
2 months and had no problems. We are a large Pharma, our lab is all Vet
Path.
There are at least 21 Vet schools in the US and a State Diagnostic lab
in every State in the country.
My HT goes back to 1982 and my HTL and IHC are from the 2003 and 2005.
The VIR group of the NSH is a large group of Registered techs.
We have always been covered under the same ASCP. There is no
distinction between human and animal. In fact, when grading the
practical (that was discontinued) in the last 10 years or so the ASCP
always had a vet path and histo tech from VIR on the grading committee.
I believe Liz was a grader.
You should call ASCP again and set them strait, maybe I will!
Mary Gessford BA,HT,HTL(ASCP)IHC
Scientist II Supervisor
Anatomic Pathology
Schering-Plough
Summit, NJ
908-473-4358
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Friday, June 06, 2008 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sally's Friday Fume
Okay, I finally have something to Fume about. I am beginning to train a
person to become a certified histologist. She is a certified veterinary
tech with an Associates Degree and the appropriate number of
chemistry/biology hours. I inquired of ASCP about her eligibility (after
we finish the training period) to take the HT exam. According to ASCP,
as a vet diagnostic lab, we do not meet the ASCP requirements for
eligibility because we are not accredited by CAP, JCAHO/AABB and do not
have a board-certified (read "human") pathologist/medical scientist
(hmmm? Could one of our veterinary pathologists fulfill the description
of "medical scientist"??). Surely there are other persons working in a
veterinary pathology lab that would be interested in certification, one
would think. What are they to do? This frosts my petunias and I'd like
to know if anyone has any experience or input on this. But - hey! - some
of my fluids/solutions in gallon/liter containers seem to have been
improved so they don't drip, so maybe someone out there IS listening
(that was my first Friday Hour of Fuming subject). Hasta lumbago.
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************
This message and any attachments are solely for the intended recipient.
If you are not the intended recipient, disclosure, copying, use or
distribution of the information included in this message is prohibited
-- Please immediately and permanently delete.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Fri, 6 Jun 2008 15:29:28 -0400
From: "Della Speranza, Vinnie"
Subject: [Histonet] RE: Veterinary Histologist Certification
Eligibility
To: "'Breeden, Sara'" ,
"histonet@lists.utsouthwestern.edu"
Cc: "'marilynng@EARTHLINK.NET'"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hi Sara,
I certainly understand your frustration and I'm in agreement with those who've responded to your original message that the very last thing we wish to do is discourage individuals who wish to become certified. I am copying Marilyn Gamble who is the NSH representative to the Board of Registry so that she can clarify if there has been any misunderstanding during this discussion on Histonet and likewise can represent the issue to the BOR.
In the days when the practical was in place, it may have been appropriate for the technician/technologist to receive guidance for the selection of appropriate tissues to satisfy the exam requirements. However, I seem to recall grading slides on animal tissues which at the time I imagined came from veterinary labs.
Marilyn please "reply to all" if you can provide information that will clarify the situation. I am forwarding the earlier messages to you separately.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, June 06, 2008 2:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Veterinary Histologist Certification Eligibility
That's a mouthful! Anyway - thank you to those who responded to me. I
searched out a board-certified pathologist (non-veterinary) who works in
our building (we share a State of NM building with OMI and the State
Laboratory) and he was as astounded as I. I appreciate the feedback
about writing a letter to BOR requesting a "waiver" for a veterinary
situation and I will try that when the time comes. Our lab, in
anticipation of our new building (2010) is operating under conditional
AALVD certification but certainly not under CAP/JCHAO. I'm thinking of
asking for a definition of "medical scientist" from the ASCP. The saga
continues, but "where there's a will, there's a way". One would think
anyone who is interested in becoming a certified histologist would
receive the maximum amount of encouragement, from the employer AND the
certifying agency. Perhaps it's time for a review of the requirements
or at least of the definition of an employer's validity to provide
appropriate training, hmmm??? Mr. Della Speranza, do you have a take on
this?
It is not my goal here to fluster, annoy, or pester. It's just that I'm
beginning to see a certain amount of reluctance on the part of those
institutions whose interest should be to encourage the growth of our
profession. Now that my aim is to interest local mid-high and high
school forensic science students in a detour to the Paraffin Side, I
have a path littered with roadblocks. If our profession had the same PR
firm that the anti-tobacco industry had, we'd be up to our earlobes in
histotechs (did that come out right?).
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Fri, 06 Jun 2008 12:49:26 -0700
From: "Behnaz Sohrab"
Subject: [Histonet] SLide Holder
To:
Message-ID: <48493246.4347.0054.0@ah.org>
Content-Type: text/plain; charset=US-ASCII
Any one knows where I can buy brown/black microwave able slide holder?
Thanks,Behnaz Sohrab
------------------------------
Message: 12
Date: Fri, 06 Jun 2008 13:01:03 -0700
From: Patti Loykasek
Subject: [Histonet] IHC neg. controls (Friday rant)
To: histonet
Message-ID:
Content-Type: text/plain; charset="ISO-8859-1"
Can someone rationalize to me the practice of running a negative control for
every antibody in an IHC workup? For example, six antibodies & six negative
controls??? This makes me crazy. Just had a case, needle biopsy, where this
occurred at an outside institution, and now we donıt have enough tumor left
to run more IHC & get a diagnosis. It borders on malpractice IMO.
On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The
comment has the following (near the end):
A negative tissue control must be processed for each antibody in a given
run. Any of the following can serve as a negative tissue control:
1. Multitissue blocks. These can provide simultaneous positive
and negative tissue controls, and are considered ³best practice²
2. The positive control slide or patient test slides, if these
slides contain tissue elements that should not react with the antibody.
3. A separate negative tissue control slide.
I think it best to asses this negative tissue control on your positive
controls that should contain negative elements. Plus, use known negative
elements on the patient slides. The patient tissue is precious & these
patients have undergone procedures that have associated morbidity. I canıt
see using up patient tissue for multiple negative controls & Having the
patient have to undergo another procedure!
Ok Iıll stop now.
Patti Loykasek
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intended recipients and may contain privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are not the intended
recipient, please contact the sender by e-mail and destroy all copies of the
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.
at (206) 374-9000.
------------------------------
Message: 13
Date: Fri, 06 Jun 2008 13:49:42 -0700
From: Patti Loykasek
Subject: Re: [Histonet] IHC neg. controls (Friday rant)
To: "Morken, Tim" , histonet
Message-ID:
Content-Type: text/plain; charset="ISO-8859-1"
Hi Tim. I totally agree with you. I DO NOT think a negative should be run
for each antibody. In the comment of the ANP.2270 they even mention running
one for each antigen retrieval, but add if not then to use most aggressive
AR & they list the AR in order of aggresiveness. Running a negative for each
antibody is waste & if cited by a CAP inspector, I would appeal to CAP -
especially considering the way the comment is written. I was hoping someone
who is running a negative with each antibody could tell me their rationale
for doing this.
Patti
> Patti, It does not say you need a separate negative tissue for each antibody,
> only "A" negative control tissue. One of each block, and for each protocol
> (ie, different pretreatments or detection system) would suffice.
>
>
> Tim Morken
> Technical Support Manager
> Lab Vision Products
> Anatomical Pathology
> ThermoFisher Scientific
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek
> Sent: Friday, June 06, 2008 1:01 PM
> To: histonet
> Subject: [Histonet] IHC neg. controls (Friday rant)
>
> Can someone rationalize to me the practice of running a negative control for
> every antibody in an IHC workup? For example, six antibodies & six negative
> controls??? This makes me crazy. Just had a case, needle biopsy, where this
> occurred at an outside institution, and now we donıt have enough tumor left to
> run more IHC & get a diagnosis. It borders on malpractice IMO.
>
> On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The
> comment has the following (near the end):
>
> A negative tissue control must be processed for each antibody in a given run.
> Any of the following can serve as a negative tissue control:
>
> 1. Multitissue blocks. These can provide simultaneous positive
> and negative tissue controls, and are considered ³best practice²
> 2. The positive control slide or patient test slides, if these
> slides contain tissue elements that should not react with the antibody.
> 3. A separate negative tissue control slide.
>
> I think it best to asses this negative tissue control on your positive
> controls that should contain negative elements. Plus, use known negative
> elements on the patient slides. The patient tissue is precious & these
> patients have undergone procedures that have associated morbidity. I canıt see
> using up patient tissue for multiple negative controls & Having the patient
> have to undergo another procedure!
> Ok Iıll stop now.
>
> Patti Loykasek
>
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipients and may contain privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not the
> intended recipient, please contact the sender by e-mail and destroy all copies
> of the original message, or you may call PhenoPath Laboratories, Seattle, WA
> U.S.A.
> at (206) 374-9000.
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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------------------------------
Message: 14
Date: Fri, 6 Jun 2008 14:04:57 -0700 (PDT)
From: Larry Woody
Subject: Re: [Histonet] IHC neg. controls (Friday rant)
To: Patti Loykasek , histonet
Message-ID: <806964.24310.qm@web53606.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
There could be a legal angle to it in which case wouldn't make it rational but that is the only thing I can come up with on a Friday.
Patti Loykasek wrote: Can someone rationalize to me the practice of running a negative control for
every antibody in an IHC workup? For example, six antibodies & six negative
controls??? This makes me crazy. Just had a case, needle biopsy, where this
occurred at an outside institution, and now we donıt have enough tumor left
to run more IHC & get a diagnosis. It borders on malpractice IMO.
On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The
comment has the following (near the end):
A negative tissue control must be processed for each antibody in a given
run. Any of the following can serve as a negative tissue control:
1. Multitissue blocks. These can provide simultaneous positive
and negative tissue controls, and are considered ³best practice²
2. The positive control slide or patient test slides, if these
slides contain tissue elements that should not react with the antibody.
3. A separate negative tissue control slide.
I think it best to asses this negative tissue control on your positive
controls that should contain negative elements. Plus, use known negative
elements on the patient slides. The patient tissue is precious & these
patients have undergone procedures that have associated morbidity. I canıt
see using up patient tissue for multiple negative controls & Having the
patient have to undergo another procedure!
Ok Iıll stop now.
Patti Loykasek
This e-mail message, including any attachments, is for the sole use of the
intended recipients and may contain privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are not the intended
recipient, please contact the sender by e-mail and destroy all copies of the
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Larry A. Woody
Seattle, Wa.
------------------------------
Message: 15
Date: Fri, 6 Jun 2008 14:07:43 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] IHC neg. controls (Friday rant)
To: Patti Loykasek , histonet
Message-ID: <23683.58242.qm@web65707.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
If you detection system is the same and all the procedure is the same for the 6 Abs, except for the Abs, you will need only ONE negative control per tissue, per block, but not per Ab.
René J.
Patti Loykasek wrote:
Can someone rationalize to me the practice of running a negative control for
every antibody in an IHC workup? For example, six antibodies & six negative
controls??? This makes me crazy. Just had a case, needle biopsy, where this
occurred at an outside institution, and now we donıt have enough tumor left
to run more IHC & get a diagnosis. It borders on malpractice IMO.
On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The
comment has the following (near the end):
A negative tissue control must be processed for each antibody in a given
run. Any of the following can serve as a negative tissue control:
1. Multitissue blocks. These can provide simultaneous positive
and negative tissue controls, and are considered ³best practice²
2. The positive control slide or patient test slides, if these
slides contain tissue elements that should not react with the antibody.
3. A separate negative tissue control slide.
I think it best to asses this negative tissue control on your positive
controls that should contain negative elements. Plus, use known negative
elements on the patient slides. The patient tissue is precious & these
patients have undergone procedures that have associated morbidity. I canıt
see using up patient tissue for multiple negative controls & Having the
patient have to undergo another procedure!
Ok Iıll stop now.
Patti Loykasek
This e-mail message, including any attachments, is for the sole use of the
intended recipients and may contain privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are not the intended
recipient, please contact the sender by e-mail and destroy all copies of the
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.
at (206) 374-9000.
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------------------------------
Message: 16
Date: Fri, 6 Jun 2008 15:40:19 -0700
From: JR R
Subject: [Histonet] RE:Peroxidase blocking in frozen sections
To:
Message-ID:
Content-Type: text/plain; charset="Windows-1252"
I use Zymed's Peroxo-Block for 30-45 seconds.
Jerry Ricks
Research Scientist
University of Washington
Department of Pathology> From: JMyers1@aol.com> Date: Fri, 6 Jun 2008 15:00:57 -0400> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Peroxidase blocking in frozen sections> > 'Netters:> I'd like to know what reagents/methods people are using to block for > endogenous peroxidase in frozen sections. Any feedback that you might be willing to > offer is greatly appreciated.> Joe> > > > **************Get trade secrets for amazing burgers. Watch "Cooking with > Tyler Florence" on AOL Food. > (http://food.aol.com/tyler-florence?video=4?&NCID=aolfod00030000000002)> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_________________________________________________________________
It's easy to add contacts from Facebook and other social sites through Windows Live(tm) Messenger. Learn how.
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------------------------------
Message: 17
Date: Fri, 6 Jun 2008 18:13:43 -0700 (PDT)
From: pruegg@ihctech.net
Subject: Re: [Histonet] IHC neg. controls (Friday rant)
To: "Patti Loykasek"
Cc: histonet
Message-ID:
<1149.74.127.102.244.1212801223.squirrel@webmail.ihctech.net>
Content-Type: text/plain;charset=iso-8859-1
Patti et al,
this has been a bone of contention in IHC for some time now, the IHC
Resource Group has discussed this year after year with no viable solution
that CAP will go for, i know for a fact that many in the business save
precious tissue and run just one negative reagent control for the antibody
species and most agressive pretreatment for the batch, then take their
chances with CAP when the time comes.
Patsy
> Can someone rationalize to me the practice of running a negative control
> for
> every antibody in an IHC workup? For example, six antibodies & six
> negative
> controls??? This makes me crazy. Just had a case, needle biopsy, where
> this
> occurred at an outside institution, and now we donıt have enough tumor
> left
> to run more IHC & get a diagnosis. It borders on malpractice IMO.
>
> On the AP CAP checklist ANP.2270 Are appropriate negative controls used?
> The
> comment has the following (near the end):
>
> A negative tissue control must be processed for each antibody in a given
> run. Any of the following can serve as a negative tissue control:
>
> 1. Multitissue blocks. These can provide simultaneous
> positive
> and negative tissue controls, and are considered ³best practice²
> 2. The positive control slide or patient test slides, if these
> slides contain tissue elements that should not react with the antibody.
> 3. A separate negative tissue control slide.
>
> I think it best to asses this negative tissue control on your positive
> controls that should contain negative elements. Plus, use known negative
> elements on the patient slides. The patient tissue is precious & these
> patients have undergone procedures that have associated morbidity. I canıt
> see using up patient tissue for multiple negative controls & Having the
> patient have to undergo another procedure!
> Ok Iıll stop now.
>
> Patti Loykasek
>
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipients and may contain privileged information. Any
> unauthorized
> review, use, disclosure or distribution is prohibited. If you are not the
> intended
> recipient, please contact the sender by e-mail and destroy all copies of
> the
> original message, or you may call PhenoPath Laboratories, Seattle, WA
> U.S.A.
> at (206) 374-9000.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 18
Date: Fri, 06 Jun 2008 21:14:38 -0400
From: "Andrea Hooper"
Subject: [Histonet] pAKT
To: Histonet
Message-ID:
Content-Type: text/plain; charset=us-ascii; format=flowed
What Abs are people using for pAKT these days? The one I had working
so well in FFPE is no longer being made by Cell Signaling.
Thanks,
Andrea
--
------------------------------
Message: 19
Date: Fri, 6 Jun 2008 18:21:08 -0700 (PDT)
From: pruegg@ihctech.net
Subject: Re: [Histonet] pAKT
To: "Andrea Hooper"
Cc: Histonet
Message-ID:
<1164.74.127.102.244.1212801668.squirrel@webmail.ihctech.net>
Content-Type: text/plain;charset=iso-8859-1
cell signaling has a couple of alternative abs for pAKT they claim are
better, i believe they are rab monoclonals, i have not tried them yet.
Patsy
> What Abs are people using for pAKT these days? The one I had working
> so well in FFPE is no longer being made by Cell Signaling.
>
> Thanks,
> Andrea
> --
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 20
Date: Fri, 6 Jun 2008 19:56:13 -0600
From: "Janet Maass"
Subject: Re: [Histonet] Sally's Friday Fume
To: "Breeden, Sara" ,
Message-ID: <004201c8c841$aaba1390$0200a8c0@Janet03b999f>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hi Sara
I think this was an error on the part of the individual that you
communicated with at ASCP.
While I served on the HT/HTL Committee for two terms, I worked in veterinary
histology. Also on The Committee was a veterinary pathologist for two
terms. While owning my own veterinary histology consulting lab, I trained
several people who took there certification exam.
Currently on The Committee is someone that is working in veterinary
histology.
Janet Maass
------------------------------
Message: 21
Date: Sat, 7 Jun 2008 09:27:18 -0500
From: "Scott, Allison D"
Subject: [Histonet] Disposing of used paraffin from the tissue
processor
To:
Message-ID:
<1872B4A455B7974391609AD8034C79FC082E1D@LBEXCH01.hchd.local>
Content-Type: text/plain; charset="iso-8859-1"
Hello to all in histoland. How are other facilities disposing of used paraffin from the tissue processor. I have a box that I have lined with several red biohazrd bag and I pour all of the used paraffin in it. I let it harden, and after about a week I pull out the hard paraffin and put it in a biohazard box that has a biohazard bag in it also, close it up and it is ready for our environmental services people to pick up and discard. I would not think that this paraffin can be put into the regular trash since tissues and chemicals have been through it. Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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To the extent the information in this e-mail and any attachments contain
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------------------------------
Message: 22
Date: Sat, 7 Jun 2008 08:44:39 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] Disposing of used paraffin from the tissue
processor
To: "Scott, Allison D" ,
histonet@lists.utsouthwestern.edu
Message-ID: <136676.61962.qm@web65702.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We used to incinerate it.
René J.
"Scott, Allison D" wrote:
Hello to all in histoland. How are other facilities disposing of used paraffin from the tissue processor. I have a box that I have lined with several red biohazrd bag and I pour all of the used paraffin in it. I let it harden, and after about a week I pull out the hard paraffin and put it in a biohazard box that has a biohazard bag in it also, close it up and it is ready for our environmental services people to pick up and discard. I would not think that this paraffin can be put into the regular trash since tissues and chemicals have been through it. Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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To the extent the information in this e-mail and any attachments contain
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------------------------------
Message: 23
Date: Sat, 7 Jun 2008 09:53:23 -0700 (PDT)
From: Linda
Subject: Re: [Histonet] NSH Histology Task Analysis and
StandardsofPerformance Competen cy
To: Norm Burnham , "Rathborne, Toni"
, "Mahoney,Janice A"
, "McKnight, Tanisha"
, histonet@lists.utsouthwestern.edu
Message-ID: <30657.26774.qm@web36503.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I would be most greatfull for a copy too.
Linda Dee
Chicago, IL
----- Original Message ----
From: Norm Burnham
To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu
Sent: Friday, June 6, 2008 10:48:33 AM
Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy
Me too!
Norm Burnham
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne,
Toni
Sent: Friday, June 06, 2008 10:27 AM
To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] NSH Histology Task Analysis and
StandardsofPerformance Competen cy
As would I.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Mahoney,Janice A
Sent: Friday, June 06, 2008 11:23 AM
To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] NSH Histology Task Analysis and Standards
ofPerformance Competen cy
I'd love to have an electronic copy as well
Jan Mahoney
Alegent Health
Omaha,NE
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight,
Tanisha
Sent: Friday, June 06, 2008 10:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance
Competen cy
Hello Histo-netters:
Would anyone happen to have an electronic copy of NSH Histology Task
Analysis and The Standards of Performance/Competency that you could send
to me? I am in desperate need of these items. I've contacted NSH for
these twice, but I have gotten no response.
I'd greatly appreciate anything you could send.
Tanisha N. McKnight, HT (ASCP)
Covance CLS Indianapolis
Specimen Management, Anatomic Pathology
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End of Histonet Digest, Vol 55, Issue 13
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