In the very near future, I will be preparing whole mounts of mouse
mammary glands. The plan is to use Carnoy's fixative and to stain with
Carmine alum overnight. That part of the procedure I think I can
handle. My main concern is that afterwards, I am to embed in paraffin
for H&E staining. Has anyone out there done this before?? Does the
Carmine alum interfere with the H&E? How thick do the sections need to
be? I am used to histology on mouse thyroids, so this is a totally new
thing for me. Any help would be appreciated.
University of Southern Indiana
8600 University Blvd.
Evansville, IN 47712
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