After a lifetime of immunostaining paraffin wax sections, in my new job I am having to deal with GMA sections. Two questions, firstly, does one have to float out the sections for a minimum of 90 seconds on 0.5% ammonium hydroxide before mounting on a slide;( with the ammonaical water apparently acting as some sort of antigen retrieval step), and immunohistochemistry on GMA sections does not work if one uses PBS as opposed to TBS.
Any help/hints gratefully received.
University of Leicester.
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