Interesting,
After my saying it worked well :-) What tissues were you working with
that the morpho was poor? (So I don't try it & get the same results) We
don't use it for everything, so I guess it could be tissue dependant. I
swear it is the shiznit for these mouse lungs.
Different strokes I guess,
Amos
Message: 1
Date: Wed, 28 May 2008 10:32:33 -0500
From: Jackie M O'Connor
>
Subject: Re: [Histonet] Formalin alternative fixative for rodent and
large animal
To: stephanie.d.rivera@gsk.com
Cc: histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
In my opinion, for pharma research, there is no worthwhile formalin
substitute that will give consistent IHC results. I have tried STF
(which was discontinued) and other subs - finally went back to formalin.
With STF, the morphology of routine H+E's was horrible. I'm sure there
are differing opinions out there - after all, this is histonet.
Jackie O'
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