------ Forwarded Message
From: Patti Loykasek 
Date: Wed, 21 May 2008 09:07:30 -0700
To: AMH-HistoLab 
Subject: Re: [Histonet] removing tissue from already H and E stained slides.

Is your pretreatment for ER & PR the same? If so, there's no need to remove
a section. The slide can be stained manually. Simply pretreat the slide
(with appropriate controls of course), use a barrier pen around each
section, carefully apply each antibody - 1 per section- & perform your
detection etc.. as usual. If your H&E is on a non-adhesive slide you do run
the risk of losing your sections during pretreatment. Hope this helps.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> I have a situation where a pathologist needs an Er and PR IHC done on a
> previous H and E stained slide. The slide has two sections on it and she
> asked if one section  can be removed to another slide to perform ER on
> one slide and PR on the other. Does anyone know of a solution that will
> remove tissue safely from a slide after H and E stained and not
> interfere with IHC?
> 
> Abington Memorial Hospital
> 1200 Old York Rd
> Abington, PA. 19001
> 
> 
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