Some time ago I tried toluidinblue alone on skin to demonstrate mastcells.
It works fine, and I think (if this is your purpose) eosin would rather
disturb the clear result (nuclei blue, mastcellgranula red).
0,1% Toluidinblue in 1% acetic acid 5 min


Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Martin,
Erin
Gesendet: Mittwoch, 04. Juni 2008 15:37
An: histonet
Betreff: [Histonet] Toluidine blue and H&E

Bob Richmond asks: "I hope somebody does try this and report back to us
all."

I'll let you know how it goes - the pathologist is not working on gastric
biopsies but rather prefers toluidine blue to H&E for certain derm cases.
We're trying to give him both.  :)




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