Nadia,
I will attach the method and all the regeant needs to you privately and
where you can purchase the correct glucose. No, glucose D-(=) Glucose will
NOT work.
Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715
----- Original Message -----
From: "Gale, Nadia"
To:
Sent: Wednesday, May 14, 2008 11:05 AM
Subject: [Histonet] Glucose oxidase method for blocking endogenous
peroxidase
I've searched the archives and cannot find what I am after... I'd like
to try the glucose oxidase method for blocking endogenous peroxidase in
tissue sections for IHC.
What is a suitable replacement for the B-d(+)glucose G5250 discontinued
by Sigma? Will the D-(+)-Glucose G8270 work? This is 4% beta isomer
and 96% alpha.
Thanks for your help,
Nadia
Nadia Gale
Lead Histotechnologist
Centre for Translational and Applied Genomics (CTAG)
3427-600 West 10th Avenue
Vancouver, BC V5Z 4E6
tel 604-877-6000 ext 2426
fax 604-877-6089
ngale@bccancer.bc.ca
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