I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue.  It might be worth giving Zymed a call to find out about this kit. Kim Merriam, MA, HT(ASCP)Cambridge, MA----- Original Message ----From: Mary L Giebel/FS/VCU To: histonet@lists.utsouthwestern.eduSent: Wednesday, May 14, 2008 12:56:51 PMSubject: [Histonet] ZYMED Histostain Plus Kit on animal tissueAt a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help.Regards,Mary Giebel  _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet      
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