Re: [Histonet] Re: Histonet Digest, Vol 54, Issue 11

From:"Gayle Callis"



If you have EDTA and also have calcium present in the solution, the EDTA is 
going to chelate the calcium, removing it.  If calcium is needed in a 
solution, I don't think I would want to have EDTA in there with it.

We have use crude trypsin for digestions to avoid having to add calcium to 
make the trypsin active (per Chris van der Loos reply).  Our enzyme 
digestion with crude trypsin worked fine.

Gayle M. Callis
HTL/HT/MT(ASCP)

----- Original Message ----- 
From: "Mary Helie" 
To: 
Sent: Friday, May 09, 2008 10:15 AM
Subject: [Histonet] Re: Histonet Digest, Vol 54, Issue 11


Before I get swamped I do not know if EDTA would or would not work I
know it chelates as well- I was just familiar with CaCl2.

histonet-request@lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
>    1. RE: Bone Flap from Skull (Sebree Linda A.)
>    2. Re: Bone Flap from Skull (Rene J Buesa)
>    3. Re: Bone Flap from Skull (Geoff McAuliffe)
>    4. Re: Bone Flap from Skull (Rivka Rachel)
>    5. California licensure (Martin, Erin)
>    6. (no subject) (Kathleen Boozer)
>    7. CAP Her2Neu regs (Satterfield, Marirose)
>    8. Why use Calcium Chloride in Trypsin Antigen Retrieval?
>       (Merced Leiker)
>    9. RE: Polymer IHC Detection (Wynn, Carmen)
>   10. ERRATUM in Why use Calcium Chloride in Trypsin Antigen
>       Retrieval? (Merced Leiker)
>   11. Bone Marrow Cores (Metzger, Kenneth)
>   12. pigment in bone marrow clot sections (Mike Pence)
>   13. Epithelial specific antigen EP CAM (AnitaIBSC)
>   14. RE: pigment in bone marrow clot sections (Liz Chlipala)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 8 May 2008 12:02:34 -0500
> From: "Sebree Linda A." 
> Subject: RE: [Histonet] Bone Flap from Skull
> To: "Lott, Robert" ,
> 
> Message-ID:
> 
>
> Content-Type: text/plain; charset="us-ascii"
>
> Just off the top of my head (no pun intended) I would think an organ
> preservation solution might be reasonable assuming that your institution
> is involved in transplantation.
>
> Linda Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-3224
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott,
> Robert
> Sent: Thursday, May 08, 2008 11:58 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Bone Flap from Skull
>
>
> A neurosurgeon just brought a bone flap from a patient and wants to
> replace it 3-4 days from now...
>
> Do any of you have suggestions or recommendations for storing the bone
> flap in the lab until he needs it again???
>
>
> Need help fast!
>
>
> Thanks!
>
>
>
> Robert L. Lott, HTL(ASCP)
>
> Manager, Anatomic Pathology
>
> Trinity Medical Center / LabFirst
>
> 800 Montclair Road
>
> Birmingham, AL  35213
>
> 205-592-5388
>
> 205-592-5646 - fax
>
> robert.lott@triadhospitals.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 8 May 2008 10:15:14 -0700 (PDT)
> From: Rene J Buesa 
> Subject: Re: [Histonet] Bone Flap from Skull
> To: "Lott, Robert" ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <160753.70968.qm@web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Freeze it at -80║C
>   RenÚ J.
>
> "Lott, Robert"  wrote:
>   A neurosurgeon just brought a bone flap from a patient and wants to
> replace it 3-4 days from now...
>
> Do any of you have suggestions or recommendations for storing the bone
> flap in the lab until he needs it again???
>
>
>
> Need help fast!
>
>
>
> Thanks!
>
>
>
>
>
> Robert L. Lott, HTL(ASCP)
>
> Manager, Anatomic Pathology
>
> Trinity Medical Center / LabFirst
>
> 800 Montclair Road
>
> Birmingham, AL 35213
>
> 205-592-5388
>
> 205-592-5646 - fax
>
> robert.lott@triadhospitals.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>        ---------------------------------
> Be a better friend, newshound, and know-it-all with Yahoo! Mobile.  Try it 
> now.
>
> ------------------------------
>
> Message: 3
> Date: Thu, 08 May 2008 13:16:51 -0400
> From: Geoff McAuliffe 
> Subject: Re: [Histonet] Bone Flap from Skull
> Cc: histonet@lists.utsouthwestern.edu, "Lott, Robert"
> 
> Message-ID: <48233583.3050809@umdnj.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> You do not need help with this, you need to get far away from this.
> I can't believe your institution does not have a protocol for this!
> Are you really going to take recommendations from Histoneters who are not 
> MDs?
> I can just see you testifying at the malpractice trail (after the 
> infected/necrotic tissue causes serious
> illness or death) that someone on histonet told you it was OK to 
> ...........
> Get my drift?
> This is obvious malpractice on the surgeon's part. Run like hell.
>
> Geoff
>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott,
>> Robert
>> Sent: Thursday, May 08, 2008 11:58 AM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] Bone Flap from Skull
>>
>>
>> A neurosurgeon just brought a bone flap from a patient and wants to
>> replace it 3-4 days from now...
>>
>> Do any of you have suggestions or recommendations for storing the bone
>> flap in the lab until he needs it again???
>>
>>
>> Need help fast!
>>
>>
>> Thanks!
>>
>>
>>
>> Robert L. Lott, HTL(ASCP)
>>
>> Manager, Anatomic Pathology
>>
>> Trinity Medical Center / LabFirst
>>
>> 800 Montclair Road
>>
>> Birmingham, AL  35213
>>
>> 205-592-5388
>>
>> 205-592-5646 - fax
>>
>> robert.lott@triadhospitals.com
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>
>
>

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