Re: [Histonet] Paraffin thickness standard or measurement?

From:Rene J Buesa

  Since time immemorial section thickness determination has been an almost unattainable goal. The now defunct American Optical Company (manufacturers of the Spencer microtomes) developed a method based on an specially designed interferometer that allowed the thickness determination. They also designed a resistance to cutting method, but even they thought neither was practical in the routine laboratory.
  I used to evaluate the thickness of the section using the lymphocytes' diameter (around 7 Ám) and if there were one or several lymphocytes layers as an indirect thickness method.
  Now if you are experiencing this problem it is better to check all the moving parts of your microtome and tight those that should be tight (including the block and blades holders) in order to avoid vibrations that are usually the cause of successive thick-thin sections.
  Hope this will help you
  RenÚ J.

amy rizzo  wrote:

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