We keep our chucks in the cryostat before sectioning and have never
had this problem (cryotstat temp is -28C). It could be that fast
freezing it is the problem. When we put oct on the chuck to attach
the specimin, the oct gets into the grooves of the chuck and then
freezes because we're not using the fast freeze mode.
Also we use Tissue Tek oct, if name brand makes a difference.
If you're a cowboy and you're dragging a guy behind your horse, I bet
it would really make you mad if you looked back and the guy was
reading a magazine.
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