Re: [Histonet] Keratin-903 on Liver

From:Patti Loykasek

Hi Ken. By referring to K-903, I believe you mean cytokeratin clone 34BE12.
The CK-903 designation is actually an old catalog number, not the name of
the keratin. Dr. Allen Gown made the 34BE12 antibody about 20 years ago=2E
It's a high molecular weight keratin (CK1,5,10 & 14). Just a bit of history
for you. But on to helping with your staining issues. This keratin should
stain the bile ducts in liver. What pretreatment are you using? This
antibody usually works with either an enzyme digestion or a gentle heat
retrieval in citrate. Let me know if you need more info.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

> One of our pathologists wants us to use K-903 on liver to tag the bile duct.
> Though our procedure works great on prostate we are having trouble with it on
> the liver..any suggestions? Thanks
> Ken Metzger HTL(ASCP)
> Histology Supervisor
> ARUP Laboratories
> 500 Chipeta way
> Salt Lake City, UT 84108
> 801.583.2787 ext 3101
> - ------------------------------------------------------------------
> The information transmitted by this e-mail and any included
> attachments are from ARUP Laboratories and are intended only for the
> recipient. The information contained in this message is confidential
> and may constitute inside or non-public information under
> international, federal, or state securities laws, or protected health
> information and is intended only for the use of the recipient.
> Unauthorized forwarding, printing, copying, distributing, or use of
> such information is strictly prohibited and may be unlawful. If you
> are not the intended recipient, please promptly delete this e-mail
> and notify the sender of the delivery error or you may call ARUP
> Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
> (800) 522-2787 ext. 2100
> _______________________=5F_______________________
> Histonet mailing list

This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.

Histonet mailing list

<< Previous Message | Next Message >>