What everyone else is saying is true and you will need to check your
procedure carefully. Manual staining is a technique that is difficult
with some antibodies and especially when working with a large slide
One of the things that I did was eliminate using a pap pen. I used 2
X 2 strips of parafilm that I cut in strips of 1 X 2 and layed them on
the tissue/slide after the reagent has been applied. To remove I
dipped the slides in buffer and the film would slide right off.
Good luck. I did IHC manually for a long time and it is not always easy.
On 5/9/08, Karla Arrington wrote:
> I do Immuno's by hand and I am getting background DAB precipitate on the inside circle of my sections.
> What is causing this and how do I get rid of it?
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