Re: [Histonet] Celestine Blue Procedure

From:Rene J Buesa

  The trick with celestine blue is the formula you use to prepare the staining solution.
  Gray prefers (and I have also used years ago) one that is as follows:
  water -- 100mL + ferric alum -- 2.5g + celestin blue B-- 0.5g + glycerol -- 14 mL + sulfuric acid -- 2 mL.
  Boil the dye in the alum solution (prepared first). Cool, filter and add the rest.
  Staining from 5 min to 1 hour (too long for a routine lab); will not overstain. Does not need "bluing".
  I don't think the hematoxylin will be unavailable any time soon.
  René J.

Ramona Turner  wrote:

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