RE: [Histonet] pigment in bone marrow clot sections

From:"Liz Chlipala"

I would be its formalin pigment, you need to check the pH of the
fixative.  You can get rid of it check freida's book.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504

-----Original Message-----
[] On Behalf Of Mike
Sent: Thursday, May 08, 2008 1:30 PM
Subject: [Histonet] pigment in bone marrow clot sections

I need to ask if anyone has seen this before? In our bone marrow clot
section that we stain H&E, I am seeing black precipitate that looks like
you were looking at anthracotic pigment.  This is at the same level as
the section on the slide. It is not debris it is only on the stained
section (the rest of the glass looks clean) and we fix in 10% NBF.  It
is not zinc, like we use to see in B-5 fixed tissues. We only see it in
very bloody specimens like bone marrows or blood clots.  Has any one
else see this or know what it is or how to get rid of it?
Thanks in advance,
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