Dorothy,
Regarding freezing compound coming off the chucks, this is usually a problem
if the chucks are held in the cryostat before embedding. The mounting media
does not get to the bottom of the groves and so does not stick. Start with a
warm chuck when freezing, even when making a blank build-up.
Best Regards,
Mickie
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
& Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX 509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com
Email: mickie25@netzero.net
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Wednesday, May 07, 2008 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lot numbers
Does everyone keep track of each reagent lot number, including, water,
formalin, alcohols, etc. as it is opened and used? What about recycled
products? I am trying to come up with a logsheet and would appreciate
any help in this area!
Also, we have recently run into problems with our freezing compound
coming off of the chucks during frozen sectioning. Any suggestions
and/or what is the method of freezing tissue that everyone uses for
their cryostat sections? Thanks fellow histotechs for helping me with
these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!!
Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Phone: 651-254-2962
Fax: 651-254-2741
Regions Hospital is part of the HealthPartners family of care
________________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient, please be
advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is strictly
prohibited.
If you have received this e-mail in error, please immediately notify the
HealthPartners Support Center by telephone at (952) 967-6600. You will be
reimbursed for reasonable costs incurred in notifying us.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|