RE: [Histonet] Lot numbers

From:"Mickie Johnson"


Regarding freezing compound coming off the chucks, this is usually a problem
if the chucks are held in the cryostat before embedding. The mounting media
does not get to the bottom of the groves and so does not stick. Start with a
warm chuck when freezing, even when making a blank build-up. 

Best Regards,
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
FAX   509-624-3926
Web: & 
-----Original Message-----
[] On Behalf Of Webb,
Dorothy L
Sent: Wednesday, May 07, 2008 10:56 AM
Subject: [Histonet] Lot numbers

Does everyone keep track of each reagent lot number, including, water,
formalin, alcohols, etc. as it is opened and used?  What about recycled
products?  I am trying to come up with a logsheet and would appreciate
any help in this area!  

Also, we have recently run into problems with our freezing compound
coming off of the chucks during frozen sectioning.  Any suggestions
and/or what is the method of freezing tissue that everyone uses for
their cryostat sections?  Thanks fellow histotechs for helping me with
these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
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